Emergence of protein-like higher-order structure and molecular recognition ability by dynamic exchange of polypeptide side chains
| Project/Area Number |
15K13705
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Polymer chemistry
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| Research Institution | Yokohama National University |
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Principal Investigator |
OYAMA Toshiyuki 横浜国立大学, 大学院工学研究院, 教授 (30313472)
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| Co-Investigator(Renkei-kenkyūsha) |
TOKORO Yuichiro 横浜国立大学, 大学院工学研究院, 助教 (80709692)
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| Research Collaborator |
INOUE Yuki
URAI Satoshi
NAKAGAWA Kazutoshi
KITAHARA Yukina
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ポリペプチド / 動的共有結合 / 高次構造 / タンパク質 |
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| Outline of Final Research Achievements |
A water-soluble polypeptide having a methyl thioester structure at the side chain was synthesized, and this polypeptide was reacted with various low-molecular-weight thiols in water. As a result, progress of dynamic bond exchange between thioester and thiol was confirmed, which indicates successful rearrangement of the polypeptide side-chain sequence in water. It was also revealed that the higher-order structure of the polypeptide changed with the progress of side-chain exchange. Furthermore, it was shown that when the exchange was carried out by using low-molecular-weight thiols having relatively high solubility in water, the resulting copolypeptide could be isolated while maintaining the side-chain composition and higher-order structure generated during the exchange reaction.
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Report
(3 results)
Research Products
(8 results)
