| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We have demonstrated laser-induced rapid and effective crystallization and crystal growth of protein molecules by focusing the trapping laser to the air-solution interface of protein solutions. When trapping laser was focused to the air-solution interface, hen egg white lysozyme solution showed huge two dimensional assembly formation, dense liquid droplets formation due to the liquid-liquid phase separation after turning-off the laser irradiation, and rapid crystal formation and its growth. Confocal Raman and polarized Raman spectroscopy under trapping laser irradiation revealed that lysozyme concentration became more than 10 times higher than starting solution and ordered orientation of lysozyme molecules under photon pressure. In ferritin case, we could induce real-time crystallization of this protein under trapping laser irradiation from the focal spot.
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