Fluorescent memory proteins

• Project/Area Number: 15K13720
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Moritoshi 東京大学, 大学院総合文化研究科, 准教授 (00323501)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 環状核酸 / 蛍光 / メモリ / 細胞 / モデル小動物 / イメージング / タンパク質 / 神経細胞

Outline of Final Research Achievements

Optical imaging could be the most powerful technique available for observing spatial and temporal dynamics of molecular processes in living cells, if optical indicators for the relevant molecular processes become available. We have developed genetically encoded fluorescent probes for imaging cyclic nucleotides, based on insertion of cyclic nucleotide binding domain into green fluorescent protein (GFP).