Development of DEP device for analysis of mechanobiology that affects cell function and differentiation
| Project/Area Number |
15K13721
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
安川 智之 兵庫県立大学, 物質理学研究科, 准教授 (40361167)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞分析 / 誘電泳動 / 幹細胞 / メカノバイオロジー / バイオ分析 / マイクロデバイス / 細胞塊 |
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| Outline of Final Research Achievements |
Although mechanical stimulation to the cells is recognized as an important factor to control cellular functions, conventional methods for cell stimulation are difficult to control the intensity and timing of mechanical stimulation with simple procedures and equipment.
In this study, we investigated the effect of positive dielectrophoresis (DEP) on gene expression in mesenchymal stem cells. When applying the alternating current voltage, human bone marrow derived mesenchymal stem cells (UE7T-13) exhibited positive DEP and were compressed onto the electrode surface. Constructed device can easily control the DEP force to the cells by changing frequency. Interestingly, gene expressions of cell differentiation marker in UE7T-13 cells and mechanical stimulation-susceptible one were changed by applying positive DEP. These results suggested that gene expression in mesenchymal stem cells can be regulated by applying mechanical stimulation derived from DEP.
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Report
(3 results)
Research Products
(9 results)
