Development of a single molecule sensor with a novel method for artificial bilayer measurements.
| Project/Area Number |
15K13725
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Okayama University |
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Principal Investigator |
Ide Toru 岡山大学, 自然科学研究科, 教授 (60231148)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 単一チャネル計測 / センサー / 1分子計測 / 人工膜 / 1分子センサー / チャネル蛋白 / 単一チャネル電流計測 / バイオセンサー / イオンチャネル |
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| Outline of Final Research Achievements |
The purpose of this study was to make a novel bio-sensor using artificial bilayers formed on the surface of a gold electrode.
(1) Improvements of conventional method for bilayer formation: We have developed the method for bilayer formation at water/metal interface. The surface of a gold electrode was modified with hydrophilic polymers. A stable artificial bilayer was formed just by moving the tip through a lipid solution layer. Using this bilayer, we measured single channel current fluctuations of channel forming peptides such as gramicidin.(2) Protein incorporation: Ion channel proteins were immobilized on the surface of the gold electrode and these were inserted into the bilayer. The channels were incorporated into the bilayer almost simaltaneously with bilayer formation at the interface. We measured single channel currents of several types of human channel proteins using this method.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] A Simple Method for Ion Channel Recordings Using Fine Gold Electrode2016
Author(s)
Okuno, D., Hirano, M., Yokota, H., Onishi, Y., Ichinose, J., Ide, T.
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Journal Title
Analytical Sciences
Volume: 32
Issue: 12
Pages: 1353-1357
DOI
NAID
ISSN
0910-6340, 1348-2246
Related Report
Peer Reviewed / Open Access
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[Presentation] Single molecule measurement of ion-channel proteins. .2017
Author(s)
Toru Ide, Saki Nomura, Minako Hirano, Junnya Ichinose, Hiroaki Yokota
Organizer
The Joint symposium of 9th International Symposium on Medical, Bio- and Nano-Electronics, and 6th International Workshop on Nanostructures & Nanoelectronics
Place of Presentation
東北大学
Related Report
Int'l Joint Research / Invited
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