Research Project
Grant-in-Aid for Challenging Exploratory Research
The purpose of this study was to make a novel bio-sensor using artificial bilayers formed on the surface of a gold electrode.(1) Improvements of conventional method for bilayer formation: We have developed the method for bilayer formation at water/metal interface. The surface of a gold electrode was modified with hydrophilic polymers. A stable artificial bilayer was formed just by moving the tip through a lipid solution layer. Using this bilayer, we measured single channel current fluctuations of channel forming peptides such as gramicidin.(2) Protein incorporation: Ion channel proteins were immobilized on the surface of the gold electrode and these were inserted into the bilayer. The channels were incorporated into the bilayer almost simaltaneously with bilayer formation at the interface. We measured single channel currents of several types of human channel proteins using this method.
All 2018 2017 2016 2015
All Journal Article (3 results) (of which Peer Reviewed: 3 results, Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 4 results, Invited: 3 results) Patent(Industrial Property Rights) (1 results)
Nanoscale
Volume: 10 Issue: 8 Pages: 4036-4040
10.1039/c7nr08098k
Insect Biochemistry and Molecular Biology
Volume: 87 Pages: 100-106
10.1016/j.ibmb.2017.06.015
Analytical Sciences
Volume: 32 Issue: 12 Pages: 1353-1357
10.2116/analsci.32.1353
130005433385
