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Monitoring of association of cell membrane receptors based on the resonance energy transfer through cell membrane

Research Project

Project/Area Number 15K13728
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionKyushu Institute of Technology

Principal Investigator

Sueda Shinji  九州工業大学, 大学院情報工学研究院, 准教授 (00325581)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsタンパク質間相互作用 / 細胞膜レセプター / 共鳴エネルギー移動 / テルビウム錯体 / GFP / FRET
Outline of Final Research Achievements

In the present study, we attempted to construct a system for monitoring association of cell membrane receptors based on the resonance energy transfer through cell membrane by labeling the receptors with donor and acceptor dyes on the outer and inner surface of cell membrane, respectively. Specifically, bradykinin B2 receptor (B2R) was chosen as a model receptor and it was labeled with a terbium complex and GFP on the outer and inner surface of cell membrane, respectively. With this system, we obtained data suggesting the association of B2R by monitoring the signal based on energy transfer from the terbium complex to GFP.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2016 2015

All Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Presentation] Time-resolved luminescence assays for biomolecules using biotin ligase conjugated with a Tb3+ complex2016

    • Author(s)
      末田慎二
    • Organizer
      Rare Earths 2016
    • Place of Presentation
      北海道大学札幌キャンパス(北海道札幌市)
    • Year and Date
      2016-06-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Monitoring of self-association of angiotensin II type 1 receptor based on the resonance energy transfer from luminescent Tb(III) complex to GFP2016

    • Author(s)
      高瀬慎也、池田知弘、末田慎二
    • Organizer
      Interdisciplinary Medical, Dental and Soft-material Researches on the move -Showcase Review in Kitakyushu-
    • Place of Presentation
      福岡県北九州市
    • Year and Date
      2016-01-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] テルビウム錯体から蛍光タンパク質への共鳴エネルギー移動を利用した細胞膜受容体間の相互作用解析系の構築2016

    • Author(s)
      村和真、高瀬慎也、末田慎二
    • Organizer
      第23回日本生物工学会九州支部飯塚大会(2016)
    • Place of Presentation
      九州工業大学飯塚キャンパス(福岡県飯塚市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] テルビウム錯体から蛍光タンパク質への共鳴エネルギー移動を利用したアンジオテンシンII受容体の会合挙動の解析2015

    • Author(s)
      高瀬慎也、池田知弘、末田慎二
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 時間分解FRETを利用したアンジオテンシンII受容体の会合挙動の解析2015

    • Author(s)
      高瀬慎也、池田知弘、末田慎二
    • Organizer
      第33回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      熊本県上天草市
    • Year and Date
      2015-07-24
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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