N-Terminally fluorescent-labeled antibodies that show fluorescence change upon antigen-binding
| Project/Area Number |
15K13739
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
Hohsaka Takahiro 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (30263619)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 抗体 / イムノアッセイ / 蛍光 |
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| Outline of Final Research Achievements |
In this study, a novel method was developed for producing N-terminally fluorescent-labeled IgG antibodies that show antigen-dependent fluorescence change. By reacting fluorescent-labeled aldehyde with IgG antibody under weakly acidic condition, the fluorescent group was specifically attached at the N terminus, and the resulting N-terminally fluorescent-labeled antibody showed fluorescence intensity changed upon the antigen binding. In addition, it is possible to synthesize dual-labeled fluorescence-responsive antibody that can detect the antigen-binding as a change in fluorescence intensity ratio, and to detect tagged proteins expressed in a cell-free translation system in a real-time manner.
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Report
(4 results)
Research Products
(21 results)
