| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Using solid-phase peptide synthesis (SPPS) and native chemical ligation (NCL), thirteen 15N-labeled amino acids were inserted at specific positions of the protein backbone, while intentionally varying the enrichment of 15N atoms. This method enabled us to assign 1H-15N signals of synthetic glycoproteins even the same type of amino acid based on the intensities of 1H-15N HSQC signals. We synthesized three kinds of glycoproteins bearing an oligosaccharide of either a bi-antennary complex-type or a high-mannose-type and non-glycosylated one. Our NMR experiments showed that glycosylation at the native glycosylation positions did not disturb protein conformation. However, temperature-varied circular dichroism (CD) spectra and T1 values indicated that oligosaccharides appeared to inhibit protein fluctuation.
We also demonstrated novel semisynthesis of tribrached complex type oligosaccharide which can prepared within 10 conversion steps from the isolated biantennary oligosaccharide.
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