Development of novel radical enzymes bearing unusual protein-derived cofactor
Project/Area Number |
15K13743
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
|
Research Institution | Osaka University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 人工金属酵素 / 翻訳後化学修飾 / ラジカル酵素 |
Outline of Final Research Achievements |
Galactose oxidase and copper amine oxidase have the protein-derived redox-active organic cofactors generated by post-translational modification near the catalytic active center. There are several lines of evidences that the copper ion plays an important role in the formation of these cofactors. The copper-substituted variants of cupin proteins have been prepared by using an E. coli expression system with a copper-containing medium. Furthermore, we introduced Tyr and/or Cys residues into several site surrounding copper center and confirmed the formation of Tyr-Cys by spectroscopic method as well as X-ray crystal structural analysis.
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Report
(3 results)
Research Products
(12 results)