Development of a method for imaging RNA digestion in cells and its application for studying stress response

• Project/Area Number: 15K13746
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Okayama University
• Principal Investigator: Ohtsuki Takashi 岡山大学, 自然科学研究科, 教授 (80321735)
• Co-Investigator(Kenkyū-buntansha): 渡邉 和則 岡山大学, 自然科学研究科, 助教 (70602027)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 核酸関連化学 / RNA分解 / ストレス応答 / tRNA / FCS / RNA / 分解速度 / 蛍光相関分光法 / 動物細胞

Outline of Final Research Achievements

Localization change and/or degradation of RNAs have been known to associated with various cellular events. In this study, a method to observe degradation of a fluorescently labeled RNA in each place in a cell by fluorescence correlation spectroscopy. We attempted to observe heat stress-induced tRNA degradation in mammal cells by this method.

Research Products

• [Int'l Joint Research] Harbin Engineering University(中国)
• [Journal Article] Detection of small, highly structured RNAs by molecular beacons. (2017)
  • Author(s): Jin Li, Chengzhen Xu, Naotaka Shimada, Yuichi Miyoshi, Kazunori Watanabe, Wang Cong, Takashi Ohtsuki
  • Journal Title: Analytical Methods Volume: 印刷中 Issue: 20 Pages: 2971-2976
  • DOI: 10.1039/c7ay00341b
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phototriggered protein syntheses by using (7-diethylaminocoumarin-4-yl)methoxycarbonyl-caged aminoacyl tRNAs. (2016)
  • Author(s): Takashi Ohtsuki, Shigeto Kanzaki, Sae Nishimura, Yoshio Kunihiro, Masahiko Sisido, Kazunori Watanabe
  • Journal Title: Nature Communication Volume: 7 Issue: 1 Pages: 12501-12501
  • DOI: 10.1038/ncomms12501
  • NAID: 120005844165
  • Peer Reviewed / Open Access
• [Presentation] 熱ストレス依存的な核内ストレス構造体形成機構の解析 (2016)
  • Author(s): 岡田真実、山本理沙子、渡邉和則、大槻高史
  • Organizer: 第39回分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] Xrn2活性化メカニズムの解明 (2016)
  • Author(s): 内田伸哉、渡邉和則、大槻高史
  • Organizer: RNAフロンティアミーティング2016
  • Place of Presentation: 山形
  • Year and Date: 2016-08-31
• [Presentation] 熱ストレスによる核内ストレス構造体形成機構の解明 (2016)
  • Author(s): 渡邉和則、岡田真実、山本理紗子、大槻高史
  • Organizer: 第89回日本生化学会
  • Place of Presentation: 仙台
• [Presentation] Molecular beacons for detecting tRNA. (2016)
  • Author(s): Yuichi Miyoshi, Kazunori Watanabe, Hiromu Kashida, Hiroyuki Asanuma, Takashi Ohtsuki
  • Organizer: 26th tRNA workshop
  • Place of Presentation: Jeju (Korea)
  • Int'l Joint Research
• [Presentation] The degradation of initiator tRNA depended on the cell cycle under heat stress. (2016)
  • Author(s): Kazunori Watanabe, Yusuke Umemoto, Akihisa Takahashi, Kenichi Ijiri, Takashi Ohtsuki
  • Organizer: 26th tRNA workshop
  • Place of Presentation: Jeju (Korea)
  • Int'l Joint Research
• [Presentation] RNAの分解と寿命の解明 (2015)
  • Author(s): 大西諒、中林孝和、渡邉和則、大槻高史
  • Organizer: BMB2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] 熱ストレスによる開始tRNAMetは細胞周期に依存して分解促進している (2015)
  • Author(s): 渡邉和則、梅本裕介、高橋昭久、井尻憲一、大槻高史
  • Organizer: 第 17回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2015-07-15
• [Remarks]
  • URL: http://www.okayama-u.ac.jp/user/ohtsuki/index.htm