Development of a method for imaging RNA digestion in cells and its application for studying stress response
| Project/Area Number |
15K13746
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
渡邉 和則 岡山大学, 自然科学研究科, 助教 (70602027)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 核酸関連化学 / RNA分解 / ストレス応答 / tRNA / FCS / RNA / 分解速度 / 蛍光相関分光法 / 動物細胞 |
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| Outline of Final Research Achievements |
Localization change and/or degradation of RNAs have been known to associated with various cellular events. In this study, a method to observe degradation of a fluorescently labeled RNA in each place in a cell by fluorescence correlation spectroscopy. We attempted to observe heat stress-induced tRNA degradation in mammal cells by this method.
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Report
(3 results)
Research Products
(11 results)
