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P-N bond formation in ribosome

Research Project

Project/Area Number 15K13751
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionUniversity of Yamanashi (2016-2017)
National Institute of Advanced Industrial Science and Technology (2015)

Principal Investigator

KAWAKAMI Takashi  山梨大学, 大学院総合研究部, 助教 (60638881)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsリボソーム / リボザイム / アミノホスホン酸 / アミノホスホニル化 / tRNA / ホスホノペプチド / 分子進化 / リン-窒素間の結合形成 / SELEX / ホスホン酸 / PUREシステム / 抗体 / プロテオミクス
Outline of Final Research Achievements

By in vitro selection using aminophosphonic acid 3, 5-dinitrobenzyl and tRNA analog-fused RNA library, we successfully identified a novel ribozyme that catalyzes tRNA-aminophosphonylation. By using aminophosphnyl-tRNA prepared by the identified ribozyme, we also successfully demonstrated that existing wild-type ribosome could not catalyze phosphono-peptide bond formation, P-N bond formation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (25 results)

All 2018 2017 2016 2015 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Peptide Selection against Reactive Small Molecular Probes for Protein Imaging and Opto-genetics by Using the DIVERSE Screening System2017

    • Author(s)
      Mizuki Yamamoto, Hiroki Suzuki, Tomohiro Iwabuchi, Yu Shimizu, Takashi Kawakami
    • Journal Title

      Peptide Science

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Directed Evolution of a Cyclized Peptoid-Peptide Chimera against a Cell-Free Expressed Protein and Proteomic Profiling of the Interacting Proteins to Create a Protein-Protein Interaction Inhibitor2016

    • Author(s)
      Takashi Kawakami, Koji Ogawa, Tomohisa Hatta, Naoki Goshima, and Tohru Natsume
    • Journal Title

      ACS Chemical Biology

      Volume: 11 Issue: 6 Pages: 1569-77

    • DOI

      10.1021/acschembio.5b01014

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] De Novo Creation of Non-enzymatically Covalent-labeling Peptide Tags for Protein Imaging by Using the DIVERSE System2016

    • Author(s)
      Miya Kurata, Naoko Yanada, Koichiro Ikai, Daiki Sakamoto, Takashi Kawakami
    • Journal Title

      Peptide Science

      Volume: 53 Pages: 227-228

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Site-specific Labeling of Peptide-tagged Proteins with Nano-sized Fluorescent Probes in Living Cells for Single-molecule Imaging2016

    • Author(s)
      Naoko Yanada, Miya Kurata, Daiki Sakamoto, Koichiro Ikai, Takashi Kawakami
    • Journal Title

      Peptide Science

      Volume: 53 Pages: 207-208

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells2015

    • Author(s)
      Takashi Kawakami, Koji Ogawa, Naoki Goshima, and Tohru Natsume
    • Journal Title

      Chemistry & Biology

      Volume: 22 Issue: 12 Pages: 1671-1679

    • DOI

      10.1016/j.chembiol.2015.10.016

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In vitro display evolution of cyclized peptidomimetics targeted to a chemically synthesized amyloid beta protein dimer2015

    • Author(s)
      Takashi Kawakami, Kazuma Murakami, Daiki Sakamoto, Mizuho Hanaki, Tohru Natsume, and Kazuhiro Irie
    • Journal Title

      Peptide Science

      Volume: 52 Pages: 323-324

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Post-translational modification mapping of collagen that binds to HSP47 by directed ligand evolution and DNLC mass spectrometry2015

    • Author(s)
      Takashi Kawakami, Shinya Ito, Naoki Goshima, Kazuhiro Nagata, and Tohru Natsume
    • Journal Title

      Peptide Science

      Volume: 52 Pages: 321-322

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Directed Evolution of tRNA-Aminophosphonating Ribozymes for Ribosomal Phosphono-Peptide Synthesis2015

    • Author(s)
      Takashi Kawakami
    • Journal Title

      Peptide Science

      Volume: 51 Pages: 337-338

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] Peptide Selection against Reactive Small Molecular Probes for Protein Imaging and Opto-genetics by Using the DIVERSE Screening System2017

    • Author(s)
      山本美月、鈴木宏輝、岩渕智宏、川上隆史
    • Organizer
      第54回ペプチド討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ChemistryからBiologyへの展開-DIVERSEスクリーニングシステムの開発とバイオイメージングへの応用-2017

    • Author(s)
      川上隆史
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] DIVERSEシステムを用いた蛋白質ラベリング用ペプチドタグ創製とバイオイメージングへの応用2017

    • Author(s)
      川上隆史
    • Organizer
      第7回「産と学をつなぐSENRIの会」
    • Place of Presentation
      千里ライフサイエンスセンタービル(大阪府大阪市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 試験管内分子進化法を用いたPPI阻害環状Nアルキルペプチド化合物の創製2016

    • Author(s)
      川上隆史
    • Organizer
      新学術領域研究「天然物ケミカルバイオロジー」地区ミニシンポジウム~天然物ケミカルバイオロジーの更なる発展を目指して~
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2016-02-23
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] De Novo Creation of Non-enzymatically Covalent-labeling Peptide Tags for Protein Imaging by Using the DIVERSE System2016

    • Author(s)
      倉田みや、簗田奈央子、猪飼航一郎、坂本大樹、川上隆史
    • Organizer
      日本ペプチド学会第53回ペプチド討論会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Site-specific Labeling of Peptide-tagged Proteins with Nano-sized Fluorescent Probes in Living Cells for Single-molecule Imaging2016

    • Author(s)
      簗田奈央子、倉田みや、坂本大樹、猪飼航一郎、川上隆史
    • Organizer
      日本ペプチド学会第53回ペプチド討論会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Directed evolution of antibody-like peptides for proteomics2015

    • Author(s)
      Takashi Kawakami, Naoki Goshima, and Tohru Natsume
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu (USA)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 無細胞分子進化法と超高感度質量分析を用いた翻訳後修飾の網羅的解析2015

    • Author(s)
      川上隆史、伊藤進也、五島直樹、永田和宏、夏目徹
    • Organizer
      日本ペプチド学会第52回ペプチド討論会
    • Place of Presentation
      平塚中央公民館(神奈川県平塚市)
    • Year and Date
      2015-11-16
    • Related Report
      2015 Research-status Report
  • [Presentation] アミロイドβ二量体を標的とする非天然型環状ペプチドの試験管内分子進化2015

    • Author(s)
      川上隆史、村上一馬、阪本大樹、花木瑞穂、夏目徹、入江一浩
    • Organizer
      日本ペプチド学会第52回ペプチド討論会
    • Place of Presentation
      平塚中央公民館(神奈川県平塚市)
    • Year and Date
      2015-11-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 進化分子工学と有機合成化学を基盤とするペプチドツール創製システムの構築2015

    • Author(s)
      川上隆史
    • Organizer
      BioJapan2015 World Business Forum
    • Place of Presentation
      パシフィコ横山(神奈川県横浜市)
    • Year and Date
      2015-10-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質ラベリング用ペプチドタグの進化分子工学的創製システムの構築2015

    • Author(s)
      川上隆史、五島直樹、夏目徹
    • Organizer
      日本ケミカルバイオロジー学会第10回年会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2015-06-10
    • Related Report
      2015 Research-status Report
  • [Presentation] プロテオミクスアプローチによるタンパク質間相互作用阻害剤の同定2015

    • Author(s)
      川上隆史、五島直樹、夏目徹
    • Organizer
      「天然物ケミカルバイオロジー~分子標的と活性制御~」第8回公開シンポジウム
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2015-06-08
    • Related Report
      2015 Research-status Report
  • [Book] 2種類の人工塩基を有するDNAアプタマー2018

    • Author(s)
      山本美月、川上隆史
    • Publisher
      羊土社
    • Related Report
      2017 Annual Research Report
  • [Book] 細胞膜透過性ペプチド創製を指向したPUREシステムとmRNAディスプレイ法による大環状Nアルキルペプチドの超高速スクリーニング2017

    • Author(s)
      山本美月、鈴木宏輝、岩渕智宏、清水優、川上隆史
    • Publisher
      技術情報協会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 山梨大学 生命環境学部 生命工学科 川上研究室のホームページ

    • URL

      http://www.bt.yamanashi.ac.jp/modules/kenkyu/index.php?content_id=20

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Remarks]

    • URL

      http://www.molprof.jp/~kawakami/

    • Related Report
      2015 Research-status Report
  • [Remarks]

    • URL

      http://researchmap.jp/takakawa/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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