Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Outline of Final Research Achievements |
By in vitro selection using aminophosphonic acid 3, 5-dinitrobenzyl and tRNA analog-fused RNA library, we successfully identified a novel ribozyme that catalyzes tRNA-aminophosphonylation. By using aminophosphnyl-tRNA prepared by the identified ribozyme, we also successfully demonstrated that existing wild-type ribosome could not catalyze phosphono-peptide bond formation, P-N bond formation.
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