P-N bond formation in ribosome
| Project/Area Number |
15K13751
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | University of Yamanashi (2016-2017)
National Institute of Advanced Industrial Science and Technology (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リボソーム / リボザイム / アミノホスホン酸 / アミノホスホニル化 / tRNA / ホスホノペプチド / 分子進化 / リン-窒素間の結合形成 / SELEX / ホスホン酸 / PUREシステム / 抗体 / プロテオミクス |
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| Outline of Final Research Achievements |
By in vitro selection using aminophosphonic acid 3, 5-dinitrobenzyl and tRNA analog-fused RNA library, we successfully identified a novel ribozyme that catalyzes tRNA-aminophosphonylation. By using aminophosphnyl-tRNA prepared by the identified ribozyme, we also successfully demonstrated that existing wild-type ribosome could not catalyze phosphono-peptide bond formation, P-N bond formation.
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Report
(4 results)
Research Products
(25 results)
