| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
pH-Responsive near-infrared fluorescent cyanine dyes have been developed. From UV-vis absorption and fluorescence measurements, dyes showed pH-dependent equilibrium between a fluorescent open-ring structure and a non-fluorescent closed-ring structure in the various pH ranges. These behaviors depend on their nucleophilicity of substituents. The pH responsiveness is reversible in all cases. From in vitro cell culture experiments, dyes were found to show the pH-dependent enhancement of fluorescence intensively through endocytosis. PEG-grafted pH-responsive dyes accumulated in tumor sites and visualized them in in vivo optical imaging.
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