Three-dimensional manipulation and control of neuronal networking by using environment of three-dimensional dynamic stimulations upon gel-embedded space
| Project/Area Number |
15K13894
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dynamics/Control
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FENG Zhonggang 山形大学, 理工学研究科, 准教授 (10332545)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 超小型３次元振動ステージ / ３次元動的力学刺激 / iPS細胞 / 分化・成長誘導制御 / 神経細胞 / ３次元ゲル包埋培養 / ３次元ニューロンネットワーク / 再生治療 / ゲル包埋培養法 / 分化誘導 / ニューロンネットワーク / ３次元培養 / 立体培養誘導 |
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| Outline of Final Research Achievements |
Not like a conventional approach but based on the principle of mechanics, a novel small sized piezoelectric 3-D vibration stage is developed to impose dynamic stimulations on cultured living cells in order to support regenerative medicine. Together with scaffold utilizing gel-embedded 3-D culture, the device enables us to enforce 3-D micro dynamic stimulations upon the cultured neuronal networks.
Appropriately soft but structurally stable scaffold is realized by optimization of collagen-gel concentration, which provides an effective method for the gel-embedded 3-D culture of neurons differentiated from iPS cells. Enforcing systematic 3-D dynamic stimulations upon gel-embedded neuronal networks by the developed 3-D vibration stage, principle usefulness of the present study as a 3-D manipulation and control method of neuronal networking has been confirmed.
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Report
(4 results)
Research Products
(18 results)
