| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Not like a conventional approach but based on the principle of mechanics, a novel small sized piezoelectric 3-D vibration stage is developed to impose dynamic stimulations on cultured living cells in order to support regenerative medicine. Together with scaffold utilizing gel-embedded 3-D culture, the device enables us to enforce 3-D micro dynamic stimulations upon the cultured neuronal networks.
Appropriately soft but structurally stable scaffold is realized by optimization of collagen-gel concentration, which provides an effective method for the gel-embedded 3-D culture of neurons differentiated from iPS cells. Enforcing systematic 3-D dynamic stimulations upon gel-embedded neuronal networks by the developed 3-D vibration stage, principle usefulness of the present study as a 3-D manipulation and control method of neuronal networking has been confirmed.
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