Photopolymerization-based 3D Single Cell Spotter for Reconstruction of Tissue Array
Project/Area Number |
15K13910
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Intelligent mechanics/Mechanical systems
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Research Institution | Toyohashi University of Technology |
Principal Investigator |
nagai Moeto 豊橋技術科学大学, 工学(系)研究科(研究院), 講師 (00580557)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 細胞3Dプリンタ / 単一細胞 / ノズルアレイ / 微小液滴生成 / 多点光照射 / 光硬化性樹脂 / 3Dプリンタ / 光硬化 / バイオプリンティング / 可変開口 / 液滴生成 / 多層マイクロ流体デバイス |
Outline of Final Research Achievements |
Our objective is to assemble a 3D tissue with a single cell spotter. This study aims to achieve high throughput single cell printing. We made the essential part of a cell spotter. Adjustable openings were composed of SU-8 vertical openings and PDMS pneumatic valves. We fabricated a nozzle structure and designed flow channels to trap single cells on the openings by calculating flow ratio through microchannels. The structure was assembled from a two-step SU-8 structure and PDMS channel. These fundamental fabrication technologies enable the patterning of single cells on a substrate. Photocurable resin droplets were generated in a microchannel and its properties were characterized.
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Report
(3 results)
Research Products
(16 results)