Photopolymerization-based 3D Single Cell Spotter for Reconstruction of Tissue Array

• Project/Area Number: 15K13910
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Toyohashi University of Technology
• Principal Investigator: nagai Moeto 豊橋技術科学大学, 工学(系)研究科(研究院), 講師 (00580557)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 細胞3Dプリンタ / 単一細胞 / ノズルアレイ / 微小液滴生成 / 多点光照射 / 光硬化性樹脂 / 3Dプリンタ / 光硬化 / バイオプリンティング / 可変開口 / 液滴生成 / 多層マイクロ流体デバイス

Outline of Final Research Achievements

Our objective is to assemble a 3D tissue with a single cell spotter. This study aims to achieve high throughput single cell printing. We made the essential part of a cell spotter. Adjustable openings were composed of SU-8 vertical openings and PDMS pneumatic valves. We fabricated a nozzle structure and designed flow channels to trap single cells on the openings by calculating flow ratio through microchannels. The structure was assembled from a two-step SU-8 structure and PDMS channel. These fundamental fabrication technologies enable the patterning of single cells on a substrate. Photocurable resin droplets were generated in a microchannel and its properties were characterized.

Research Products

• [Journal Article] Integration of microorganism Vorticella convallaria and poly (ethylene glycol) diacrylate hydrogel for biohybrid systems (2016)
  • Author(s): Moeto Nagai*, Kei Kato, Takayuki Shibata
  • Journal Title: Mechanical Engineering Letters Volume: 2 Issue: 0 Pages: 16-00445
  • DOI: 10.1299/mel.16-00445
  • NAID: 130005249802
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Underwater motion of hydrogel microstructure by optofluidic lithography studied with gap control and object holding platform (2016)
  • Author(s): Moeto Nagai*, Kei Kato, Takayuki Shibata
  • Journal Title: Microelectronic Engineering Volume: 164 Pages: 108-114
  • DOI: 10.1016/j.mee.2016.08.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization of light-controlled Volvox as movable microvalve element assembled in multilayer microfluidic device (2015)
  • Author(s): Moeto Nagai, Michihito Oguri, and Takayuki Shibata
  • Journal Title: Japanese Journal of Applied Physics Volume: 54 Issue: 6 Pages: 067001-067001
  • DOI: 10.7567/jjap.54.067001
  • NAID: 210000145211
  • Peer Reviewed
• [Journal Article] Dynamic formation of a microchannel array enables kinesin-driven microtubule transport between separate compartments on a chip (2015)
  • Author(s): K. Fujimoto, M. Nagai, H. Shintaku, H. Kotera, R. Yokokawa
  • Journal Title: Lab on a chip Volume: 15 Issue: 9 Pages: 2055-2063
  • DOI: 10.1039/c5lc00148j
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development and Characterization of Hollow Microprobe Array as a Potential Tool for Versatile and Massively Parallel Manipulation of Single Cells (2015)
  • Author(s): M. Nagai, K. Oohara, K. Kato, T. Kawashima, T. Shibata
  • Journal Title: Biomedical Microdevices Volume: 17 Issue: 2 Pages: 41-41
  • DOI: 10.1007/s10544-015-9943-z
  • Peer Reviewed
• [Presentation] Single cell processing microsystems for high throughput function analysis of cells (2017)
  • Author(s): Moeto NAGAI
  • Organizer: IEEE NEMS 2017
  • Place of Presentation: University of California Los Angeles
  • Year and Date: 2017-04-09
  • Int'l Joint Research / Invited
• [Presentation] 超並列単一細胞プリンタの開発 ―可変開口を用いた細胞アセンブリ技術の確立― (2017)
  • Author(s): 河原田 翔，平塚翔太，佐藤省吾，永井萌土，柴田隆行
  • Organizer: 精密工学会 2017年度春季大会
  • Place of Presentation: 慶應義塾大学矢上キャンパス
  • Year and Date: 2017-03-13
• [Presentation] 超並列単一細胞プリンタを実現するための光硬化性マイクロ液滴操作技術の開発 (2017)
  • Author(s): 佐藤省吾，河原田翔，平塚翔太，永井萌土，柴田隆行
  • Organizer: ライフサポート学会フロンティア講演会
  • Place of Presentation: 芝浦工業大学 豊洲キャンパス
  • Year and Date: 2017-03-10
• [Presentation] Massively Parallel Single-Cell Printer DMD-based Optical System for Single Cell Immobilization (2016)
  • Author(s): Shota Hiratsuka, Sho Kawaharada, Shogo Sato, Moeto Nagai, and Takayuki Shibata
  • Organizer: The Irago Conference 2016
  • Place of Presentation: University of Electro-Communications
  • Year and Date: 2016-11-01
  • Int'l Joint Research
• [Presentation] 光造形 3D シングルセルスポッタの開発（第 1 報）可変開口を用いた細胞配置技術と光パターニング法の確立 (2016)
  • Author(s): 河原田 翔， 平塚翔太， 永井萌土， 柴田隆行
  • Organizer: 精密工学会2016年度春季大会
  • Place of Presentation: 東京理科大学 野田キャンパス
  • Year and Date: 2016-03-15
• [Presentation] 光硬化性ゲルを用いた低侵襲 3 次元細胞組織アセンブリ技術の開発 (2015)
  • Author(s): 平塚 翔太,河原田 翔,永井 萌土,柴田 隆行
  • Organizer: 第16回計測自動制御学会システムインテグレーション部門講演会（SI2015）
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-12-14
• [Presentation] 3 次元細胞組織アセンブリのための可変開口を利用した単一細胞の捕獲・脱離技術の開発 (2015)
  • Author(s): 河原田翔，永井萌土，柴田隆行
  • Organizer: 精密工学会２０１５年度秋季大会
  • Place of Presentation: 東北大学 川内北キャンパス
  • Year and Date: 2015-09-04
• [Remarks] 研究者「永井萌土」に関するウェブページ
  • URL: https://sites.google.com/site/moeton/
• [Remarks] 大学での研究に関するウェブページ
  • URL: https://www.tut.ac.jp/university/faculty/me/667.html
• [Remarks] 豊橋技術科学大学 機械工学系 マイクロ・ナノ機械システム研究室
  • URL: http://mems.me.tut.ac.jp/
• [Patent(Industrial Property Rights)] 貫通孔と流路を一体化したマイクロ流体輸送構造体およびその製造方法 (2017)
  • Inventor(s): 永井萌土、柴田隆行、河原田翔
  • Industrial Property Rights Holder: 豊橋技術科学大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-026311
  • Filing Date: 2017-02-15