On Chip Purifyication of hiPSC-derived Cells Using a Fishnet-like Mircofluidics

• Project/Area Number: 15K13911
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Osaka University (2017); Kyoto University (2015-2016)
• Principal Investigator: Liu Li 大阪大学, 医学系研究科, 特任准教授(常勤) (50380093)
• Co-Investigator(Kenkyū-buntansha): 南 一成 大阪大学, 医学系研究科, 特任准教授(常勤) (40362537)
• Co-Investigator(Renkei-kenkyūsha): KOTERA Hidetoshi 京都大学, 工学研究科, 教授 (20252471)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 幹細胞 / マイクロ加工 / ナノファイバー / 組織 / 細胞純化 / マイクロ流体デバイス / 細胞分離精製

Outline of Final Research Achievements

Human induced pluripotent stem cells (hiPSC) can be differentiated at high efficiency into cells of a targeting type but the resulted cell population has to be of high purity to avoid teratomas for clinical therapies. Herein we report a microfluidic device with integrated and surface functionalised fishnet-like structures for specific cell capture. With the help of a flow derivation surface pattern, cells in solution are forced to cross the fishnet-like structure, resulting in high efficiency and selective retention of a chosen cell population. A suspension of hiPSCs spiked in culture medium or hiPSC derived cardiomyocytes (CMs) containing medium was used for devices function validation. We found a hiPSC capture rate as high as 80% and a remarkable increase of the CM population rate in the recovered suspension without affecting the cell viability.

Research Products

• [Int'l Joint Research] East China Normal University, Shanghai(China)
• [Int'l Joint Research] Ecole Normale Superteure, Paris(France)
• [Int'l Joint Research] East China Normal University, Shanghai(China)
• [Int'l Joint Research] Ecole Normale Superieure, Paris(France)
• [Int'l Joint Research] East China Normal University, Shanghai(China)
• [Int'l Joint Research] Ecole Normale Superieure, Paris(France)
• [Journal Article] Culture Substrate Made of Elastomeric Micro-Tripod Arrays for Long-Term Expansion of Human Pluripotent Stem Cells (2017)
  • Author(s): Li, J., Zhang, F., Yu, L., Fujimoto, N., Yoshioka, M., Li, X., Shi, J., Kotera, H., Liu, L*, Chen, Y*
  • Journal Title: Journal of Materials Chemistry B. Volume: 5 Issue: 2 Pages: 236-244
  • DOI: 10.1039/c6tb02246d
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] On chip purification of hiPSC-derived cardiomyocytes using a fishnet-like microstructure (2016)
  • Author(s): Xin Li, Leqian Yu, Junjun Li, Itsunari Minami, Minako Nakajima, Yuichiro Noda, Hidetoshi Kotera, Li Liu*, Yong Chen*
  • Journal Title: Biofabrication Volume: 8 Issue: 3 Pages: 035017-035026
  • DOI: 10.1088/1758-5090/8/3/035017
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers (2016)
  • Author(s): Yadong Tang, Li Liu, Junjun Li, Leqian Yu, Francesco Paolo Ullo Severino, Li Wang, Jian Shi, Xiaolong Tu, Vincent Torre, Yong Chen
  • Journal Title: Journal of Materials Chemistry B Volume: - Issue: 19 Pages: 3305-3312
  • DOI: 10.1039/c6tb00351f
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant