| Project/Area Number |
15K13911
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Osaka University (2017)
Kyoto University (2015-2016) |
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Principal Investigator |
Liu Li 大阪大学, 医学系研究科, 特任准教授(常勤) (50380093)
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| Co-Investigator(Kenkyū-buntansha) |
南 一成 大阪大学, 医学系研究科, 特任准教授(常勤) (40362537)
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| Co-Investigator(Renkei-kenkyūsha) |
KOTERA Hidetoshi 京都大学, 工学研究科, 教授 (20252471)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 幹細胞 / マイクロ加工 / ナノファイバー / 組織 / 細胞純化 / マイクロ流体デバイス / 細胞分離精製 |
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| Outline of Final Research Achievements |
Human induced pluripotent stem cells (hiPSC) can be differentiated at high efficiency into cells of a targeting type but the resulted cell population has to be of high purity to avoid teratomas for clinical therapies. Herein we report a microfluidic device with integrated and surface functionalised fishnet-like structures for specific cell capture. With the help of a flow derivation surface pattern, cells in solution are forced to cross the fishnet-like structure, resulting in high efficiency and selective retention of a chosen cell population. A suspension of hiPSCs spiked in culture medium or hiPSC derived cardiomyocytes (CMs) containing medium was used for devices function validation. We found a hiPSC capture rate as high as 80% and a remarkable increase of the CM population rate in the recovered suspension without affecting the cell viability.
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