| Project/Area Number |
15K13921
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Power engineering/Power conversion/Electric machinery
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | ナノ秒パルス / 電界穿孔 / 大腸菌 / 生残率 / 帯電ジェット |
|---|
| Outline of Final Research Achievements |
Electropermeabilization or electroporation of the membranes can induce reversible defects of the membrane to allow direct access of external molecules into the cell. We examined the effectiveness of 8 ns full width at half maximum electric pulse with a field strength over 20 kV/cm on the permeabilization of Escherichia coli (E. coli) using a fluorescent microscope and two different stains. Nanosecond electric pulses successively permeabilized E. coli cells without damaging the living cells. The uptake ratio of propidium iodide (PI) dye was a function of pulse frequency and the ratio increased over time up to 40%. Cell viability was nearly 100% under different treating conditions.
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