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New monitoring system of 2MIB production rate of cyanobacteria using the genes occurrence analysis to develop a counter measure against the musty odor problems of reservoirs

Research Project

Project/Area Number 15K14038
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Hydraulic engineering
Research InstitutionSaitama University

Principal Investigator

ASAEDA TAKASHI  埼玉大学, 理工学研究科, 教授 (40134332)

Co-Investigator(Kenkyū-buntansha) 山口 雅利  埼玉大学, 理工学研究科, 准教授 (20373376)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsシアノバクテリア / カビ臭 / 2MIB / 水質改善技術 / 貯水池異種味障害 / 曝気循環 / 干し上げ対策 / シュードアナべナ / 2MIB / シュードアナベナ / アオコ対策 / 光阻害 / 貯水池管理 / 過酸化水素 / 曝気循環対策 / 貯水池 / ジメチルイソボルネオール / 富栄養化対策 / 貯水池異臭味障害 / 貯水池水質管理 / 渡良瀬貯水池 / 渡瀬貯水池
Outline of Final Research Achievements

The understanding of the environmental condition to generate musty odor was conducted for Cyanobacteria, Pseudoanabaena galeata, which generates musty odor, by analyzing GPPT and MIBS genes occurrence, in order to elucidate a new counter-treatment system against musty odor problems of water in reservoirs. There was a high correlation between these genes occurrence and the 2MIB production rate. It indicates the availability of the occurrence to predict the 2MIB production rate. Then, the method was applied to confirm the empirically developed methods.
The genes occurrence in cyanobacteria cells was decreased under the draw up bottom sediment of a reservoir, confirming the effectiveness of this counter-treatment. With increasing nitrogen concentration of water, the occurrence became larger, indicating the possible 2MIB generation in nitrogen rich water. The developed method is available to instantaneously understand the 2MIB production rate of cyanobacteria.

Academic Significance and Societal Importance of the Research Achievements

貯水池のカビ臭問題には、的確な対策がなく、例年、多額の予算がカビ臭除去のための活性炭処理の費用として費やされている。貯水池に発生するアオコは、リン濃度が高い間は、カビ臭を発生しないミクロキスティス属が主流になるのに対し、水質対策が進み、流入水のリン濃度が低下すると、シュードアナべナなどのカビ臭物質を生産する種に変化することが経験的に知られており、今後、より深刻な問題になることが予想されている。そうした中、近年、カビ臭を生産する過程で必要になる遺伝子が発見され、それを用いた分析が可能になった。本研究では、その手法を、これまでの対策に適用、その有効性を示し、新しい対策の開発のための手法を得た。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Effects of elevated pressure on Pseudanabaena galeata Böcher in varying light and dark environments2018

    • Author(s)
      L.A.H.Damitha, T.Asaeda, Md Harun Rashid
    • Journal Title

      Environmental Science and Pollution Research

      Volume: Online Issue: 21 Pages: 1-9

    • DOI

      10.1007/s11356-018-2218-5

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Buoyancy limitation of filamentous cyanobacteria under prolonged pressure due to the gas vesicles collapse2017

    • Author(s)
      L.A.H Damitha, T.Asaeda
    • Journal Title

      Environmental Management

      Volume: 60 Issue: 2 Pages: 293-303

    • DOI

      10.1007/s00267-017-0875-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Buoyancy limitation of filamentous cyanobacteria under prolonged pressure due to the gas vesicles collapse2017

    • Author(s)
      H.D.L.Abeynayaka, T.Asaeda
    • Journal Title

      Environmental management

      Volume: -

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] An alternative method to improve the settleability of gas-vacuolated cyanobacteria by collapsing gas vesicles2016

    • Author(s)
      H.D.L.Abeynayaka, T.Asaeda, K.Tanaka, K.Atuszawa, Y.Kaneko, H.Nishida, S.Inada
    • Journal Title

      Water Science and Technology

      Volume: 16 Issue: 6 Pages: 1552-1560

    • DOI

      10.2166/ws.2016.068

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Potential effects of UV-A radiation on scum forming cyanobacterium Microcystis aeruginosa2016

    • Author(s)
      Helayaye Damitha Lakmali Abeynayaka, Takashi Asaeda, Yasuko Kaneko
    • Organizer
      7th EAFES International Congress
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2016-08-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] An eco-friendly method for controlling cyanobacterial blooms in lakes and reservoirs2016

    • Author(s)
      Helayaye Damitha Lakmali Abeynayaka, Takashi Asaeda, Yasuko Kaneko
    • Organizer
      7th International Conference on Water Resources and Environment Research
    • Place of Presentation
      京都府京都市
    • Year and Date
      2016-06-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Alteration of 2-methylisoborneol synthesis in Pseudanabaena galeata due to high pressure and darkness in deep water2015

    • Author(s)
      Helayaye Damitha Lakmali Abeynayaka, Yuu Shiganami, Takahiro Yoshida, Maki Kawai-Yamada, Takashi Asaeda
    • Organizer
      International Environmental Engineering Conference
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2015-10-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Hydrostatic pressure induced variations of 2-methylisobroneol and pigments in filamentous cyanobacteriam: Pseudanabaena galeatea2015

    • Author(s)
      Helayaye Damitha Lakmali Abeynayaka, Takashi Asaeda
    • Organizer
      第80回 日本陸水学会
    • Place of Presentation
      北海道函館市、北海道大学 函館キャンパス
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Pressure Induced Adverse Effects on Buoyancy Regulation of Fresh Water Cyanobacteria: Psudanabeana galeata and Microcystis aeruginosa2015

    • Author(s)
      H.D.L. Abeynayaka, K. Tanaka, K. Atsuzawa, Y. Kaneko, T. Asaeda
    • Organizer
      第19回 応用生態工学講演会
    • Place of Presentation
      福島県郡山市、日本大学 郡山キャンパス
    • Year and Date
      2015-09-10
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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