Development of polymerase without cofactor

• Project/Area Number: 15K14236
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: Akihiko Kondo 神戸大学, 科学技術イノベーション研究科, 教授 (40205547)
• Co-Investigator(Kenkyū-buntansha): 田中 勉 神戸大学, 工学研究科, 准教授 (90436551)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞表層工学

Outline of Final Research Achievements

Most of polymerase using basic compounds as a substrate requires co-factors such as acetyl-CoA. Here, we tried to develop an novel enzyme working without cofactors. Protein expression systems have been established and expanded into cell surface display system. However, the activity of displayed enzyme was not enough for further evolution, another strategy should be needed to improve protein function as well as optimization of protein expression and directed evolution.