| Project/Area Number |
15K14240
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Sugiura Shinji 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (10399496)
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| Co-Investigator(Kenkyū-buntansha) |
高木 俊之 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (10248065)
柳川 史樹 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 研究員 (50645877)
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| Co-Investigator(Renkei-kenkyūsha) |
YANAGAWA Fumiki 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 研究員 (50645877)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 細胞 / ゲル / 血管 / 微細構造 / 光分解性ゲル / 光造型 / 多光子励起 / パターン / 光造形 |
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| Outline of Final Research Achievements |
We synthesized a click-crosslinkable and photocleavable crosslinker to prepare a photodegradable hydrogel. The preparation conditions of the photodegradable hydrogel with various basepolymers and the concentration of the crosslinker were investigated and evaluated from the viewpoints of hydrogel forming properties, hydrogel decomposition characteristics, and cell adhesiveness. We found conditions that vascular endothelial cells have good adhesiveness and good patterned degradation. In addition, we performed multiphoton fabrication in the photodegradable hydrogel by introducing photodegradable hydrogel into the microchannel and scanning the femtosecond laser onto the hydrogel. We considered that introduction of vascular endothelial cells into the formed luminal structure makes it possible to construct a blood vessel like tissue.
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