| Project/Area Number |
15K14242
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Central Research Institute of Electric Power Industry |
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Principal Investigator |
Matsumoto Norio 一般財団法人電力中央研究所, 環境科学研究所, 上席研究員 (40371512)
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| Co-Investigator(Kenkyū-buntansha) |
平野 伸一 一般財団法人電力中央研究所, 環境科学研究所, 主任研究員 (20392748)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 鉄酸化細菌 / 電気培養 / 二酸化炭素 / 乳酸 / メタボローム解析 / 遺伝子組み換え / 還元力 |
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| Outline of Final Research Achievements |
In this research, we aimed to prove a novel technical concept to convert carbon dioxide into valuable substances by using electricity as a reducing power for iron oxidizing bacteria, Acidithiobacillus ferrooxidans. A recombinant strain of A. ferrooxidans that were constructed by introducing the lactate dehydrogenase gene derived from Lactococcus lactis was cultivated by providing electron to cells using electrochemical cultivation technology. Although a lactate accumulation was hardly observed during cultivation without electrochemically providing electrons, significant increases of lactate accumulation was observed with providing electrons by electrochemical cultivation method. Therefore, the novel concept of electrochemically conversion of carbon dioxide by using Acidithiobacillus ferrooxidans was proved.
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