Is genomic instability causally related to memory instability?

• Project/Area Number: 15K14306
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Tohoku University
• Principal Investigator: Yamamoto Daisuke 東北大学, 生命科学研究科, 教授 (50318812)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
• Keywords: Btkチロシンキナーゼ

Outline of Final Research Achievements

The Tec family non-receptor-type tyrosine kinase Btk29A in Drosophila was found to induce transposon mobilization when mutated. Transposon mobilization is a major mechanism to induce genomic instability. We discovered that the Btk29A mutation also induces a decifit in courtship memory in male flies. This supports the notion that genomic instabilty and memory instability are related to each other. To obtain insights into the mechanism of action of Btk29A in these processes, we are conducting genome wide screens for phenotypic modifiers of Btk29A mutants.

Research Products

• [Journal Article] Quantitative analysis of visually induced courtship elements in Drosophila subobscura. (2017)
  • Author(s): Higuchi, T., Kohatsu, S., Yamamoto, D.
  • Journal Title: J. Neurogenet. Volume: 印刷中 Issue: 1-2 Pages: 1-9
  • DOI: 10.1080/01677063.2017.1290613
  • Peer Reviewed
• [Journal Article] Serotonergic neuronal death and concomitant serotonin deficiency curb copulation ability of Drosophila platonic mutants. (2016)
  • Author(s): Yilmazer YB, Koganezawa M, Sato K, Xu J, Yamamoto D.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 13792-13792
  • DOI: 10.1038/ncomms13792
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Wolbachia protein TomO targets nanos mRNA and restores germ stem cells in Drosophila Sex-lethal mutants. (2016)
  • Author(s): Ote, M., Ueyama, M., Yamamoto, D.
  • Journal Title: Curr. Biol. Volume: 26 Issue: 17 Pages: 2223-2232
  • DOI: 10.1016/j.cub.2016.06.054
  • Peer Reviewed
• [Journal Article] Fruitless represses robo1 transcription to shape male-specific neural morphology and behavior in Drosophila. (2016)
  • Author(s): Ito, H., Sato, K., Kondo, S., Yamamoto, D.
  • Journal Title: Curr. Biol. Volume: 26 Issue: 12 Pages: 1532-1542
  • DOI: 10.1016/j.cub.2016.04.067
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The neural circuitry that functions as a switch for courtship versus aggression in Drosophila males (2016)
  • Author(s): Masayuki Koganezawa, Ken-ichi Kimura and Daisuke Yamamoto
  • Journal Title: Current Biology Volume: in press Issue: 11 Pages: 1395-1403
  • DOI: 10.1016/j.cub.2016.04.017
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Clustered regulatory interspaced short palindromic repeats (CRISPR)-mediated mutagenesis and phenotype rescue by piggyBac transgenesis in a nonmodel Drosophila species. (2016)
  • Author(s): Tanaka, R., Murakami, H., Ote, M. and Yamamoto, D.
  • Journal Title: Insect Mol. Biol. Volume: 25 Issue: 4 Pages: 12232-12232
  • DOI: 10.1111/imb.12232
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] What does the fruitless tell us about nature vs. nurture in the sexual life of Drosophila? (2016)
  • Author(s): Yamamoto, D., Kohatsu, S.
  • Journal Title: Fly Volume: - Issue: 2 Pages: 1-9
  • DOI: 10.1080/19336934.2016.1263778
  • Peer Reviewed
• [Journal Article] Requirement of the Tec family tyrosine kinase Btk29A for courtship memory in Drosophila males. (2016)
  • Author(s): Sunouchi, K. Koganezawa, M. and Yamamoto, D.
  • Journal Title: Arch. Insect Biochem. Physiol. Volume: 91 Issue: 3 Pages: 165-174
  • DOI: 10.1002/arch.21316
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Brain insulin cells sense cold via Gustatory Receptor 28b for the control of diapause in Drosophila. (2017)
  • Author(s): Hara, H., Ojima, N., Yaoita, S., Ito, H., Yamamoto, D.
  • Organizer: 58th Annual Drosophila Research Conference
  • Place of Presentation: Town & Country Resort & Convention Center, San Diego, USA
  • Year and Date: 2017-03-29
  • Int'l Joint Research
• [Presentation] Brains insulin cells and the control of diapause. (2017)
  • Author(s): Yamamoto, D.
  • Organizer: Invertebrate Neuropeptide Conference 2017
  • Place of Presentation: The Patios de Cafayate Hotel, Cafayate, Argentina
  • Year and Date: 2017-02-17
  • Int'l Joint Research
• [Presentation] セロトニンによるショウジョウバエ経験依存的求愛行動の調節とその神経基盤 (2016)
  • Author(s): 小金澤雅之, 山元大輔.
  • Organizer: 新学術領域「適応回路シフト」第4回領域会議
  • Place of Presentation: 学術総合センター, 東京
  • Year and Date: 2016-12-19
• [Presentation] Nature vs. nurture in fly courtship. (2016)
  • Author(s): 山元大輔.
  • Organizer: Foreign Expert Project Seminar at Plant Protection Research Institute, Guangzhou, China
  • Place of Presentation: 広東省農業科学院, 植物保護研究所、中国
  • Year and Date: 2016-11-14
  • Invited
• [Presentation] Sexually dimorphic neural circuity that functions as a switch for courtship versus aggression in Drosophila. (2016)
  • Author(s): Koganezawa, M., Yamamoto, D.
  • Organizer: The 22nd International Congress of Zoology. The 87th meeting of Zoological Society of Japan. (Joint events)
  • Place of Presentation: 沖縄科学技術大学院大学. 沖縄
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] ニーマンピック病C型におけるオートファジー不全を改善する因子の解析 (2016)
  • Author(s): 柳澤比呂子, 秋山けい子, 石井智裕, 渡部和彦, 河上江美子, 遠藤堅太郎, 山元大輔, 衞藤義勝.
  • Organizer: 第58回日本先天代謝異常学会
  • Place of Presentation: 京王プラザホテル, 東京
  • Year and Date: 2016-10-27
• [Presentation] ショウジョウバエにおける求愛行動の種特異性を生み出す神経基盤の探索 (2016)
  • Author(s): 田中良弥、村上日向、佐藤耕世、山元大輔.
  • Organizer: 行動遺伝学研究会
  • Place of Presentation: 国立遺伝学研究所, 静岡
  • Year and Date: 2016-10-13
• [Presentation] ショウジョウバエfruitless変異体の雄同士の求愛と遺伝子-環境相互作用 (2016)
  • Author(s): 山元大輔.
  • Organizer: 行動遺伝学研究会
  • Place of Presentation: 国立遺伝学研究所,静岡
  • Year and Date: 2016-10-13
  • Invited
• [Presentation] 求愛と攻撃の相反性を実現するショウジョウバエ脳内神経回路 (2016)
  • Author(s): 小金澤雅之, 山元大輔.
  • Organizer: 行動遺伝学研究会
  • Place of Presentation: 国立遺伝学研究所, 静岡
  • Year and Date: 2016-10-13
  • Invited
• [Presentation] Phenotypic analysis of a fruitless mutant and CRISPR-mediated transgenesis in D. subobscura. (2016)
  • Author(s): Tanaka, R., Murakami, H., Sato, K., Yamamoto, D.
  • Organizer: 第12回日本ショウジョウバエ研究会
  • Place of Presentation: 立教大学, 東京
  • Year and Date: 2016-09-09
• [Presentation] Drosophila subobscuraに特異的な性行動解析のための体細胞モザイクシステムの作成 (2016)
  • Author(s): 村上日向, 田中良弥, 樋口智大, 伊藤弘樹, 佐藤耕世, 山元大輔.
  • Organizer: 日本遺伝学会第88回大会
  • Place of Presentation: 日本大学国際関係学部北口校舎,静岡
  • Year and Date: 2016-09-07
• [Presentation] Central brain neurons controlling courtship and aggression in Drosophila melanogaster. (2016)
  • Author(s): Koganezawa, M., Yamamoto, D.
  • Organizer: 第38回日本比較生理生化学学会
  • Place of Presentation: 玉川大学, 東京
  • Year and Date: 2016-09-02
• [Presentation] Drosophila subobscuraにおけるfruitless変異体の作製と遺伝学的ツールの適用～行動の種間差を生み出す神経基盤を探る (2016)
  • Author(s): 田中良弥, 村上日向, 佐藤耕世, 山元大輔.
  • Organizer: 日本進化学会第18回大会
  • Place of Presentation: 東京工業大学大岡山キャンパス, 東京
  • Year and Date: 2016-08-25
• [Presentation] Identification of the fruitless gene cis element that induces male-specific muscle in Drosophila. (2016)
  • Author(s): Nakamura, Y., Ito, H., Yamamoto, D.
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜, 神奈川
  • Year and Date: 2016-07-24
• [Presentation] ショウジョウバエの攻撃と求愛の切替を行う脳内神経回路 (2016)
  • Author(s): 小金澤雅之, 山元大輔.
  • Organizer: 日本動物学会平成28年度東北大会
  • Place of Presentation: 福島医科大学, 福島
  • Year and Date: 2016-07-23
• [Presentation] The circuit mechanism for courtship behavior in Drosophila melanogaster. (2016)
  • Author(s): Yamamoto, D.
  • Organizer: 第8回アジアオセアニア比較内分泌学会議
  • Place of Presentation: 高麗大学校医科大学. 韓国
  • Year and Date: 2016-06-20
  • Int'l Joint Research / Invited
• [Book] Male fruit fly’s courtship and its double control by the fruitless and doublesex genes. In: Animal Models of Behavior Genetics. (2016)
  • Author(s): Yamamoto, D.
  • Publisher: Springer