Representation of motor learning and motor timing in the cerebellum

• Project/Area Number: 15K14312
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: University of Yamanashi
• Principal Investigator: KITAMURA Kazuo 山梨大学, 総合研究部, 教授 (60423159)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: システム神経生理学 / 小脳 / 運動制御 / 運動学習

Outline of Final Research Achievements

Climbing fiber (CF) inputs to Purkinje cells carry error and/or timing signals for motor control and learning. CFs separately project to longitudinal clusters of Purkinje cells (cerebellar zones), which are characterized by the expression of aldolase C. We investigated the representation of CF inputs to the distinct cerebellar zones in Crus II of the cerebellar hemisphere by using two-photon calcium imaging in mice learning a go/no-go auditory discrimination task. CF inputs to the lateral aldolase C-positive zones were timed to the go cues and enhanced during the course of learning. Those to the lateral aldolase C-negative zones were timed only to the no-go cues, which declined along with learning. Those to the medial zones were longer in latency and showed lick-related representation. Our results indicate that differential and parallel processing of sensorimotor and cognitive information in the distinct cerebellar zones may collectively sophisticate goal-directed behavior.

Research Products

• [Journal Article] Dendritic spikes in sensory perception. Frontiers in Cellular Neuroscience. (2017)
  • Author(s): Manita S, Miyakawa H, Kitamura K, Murayama M
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 11 Pages: 29-29
  • DOI: 10.3389/fncel.2017.00029
  • Peer Reviewed / Open Access
• [Presentation] A novel method for assessment of reaching and grasping movements in head-fixed mice. (2017)
  • Author(s): Manita S, Ikezoe K, Kitamura K
  • Organizer: 第94回日本生理学大会
  • Place of Presentation: アクトシティ浜松（静岡県・浜松市）
  • Year and Date: 2017-03-28
• [Presentation] Representation of cerebellar climbing fiber signals during goal-directed behavior and learning. (2016)
  • Author(s): Kitamura K
  • Organizer: NIPS International Workshop “Toward elucidation of memory engram”
  • Place of Presentation: 生理学研究所（愛知県・岡崎市）
  • Year and Date: 2016-12-07
  • Int'l Joint Research / Invited
• [Presentation] Functional differences in climbing fiber inputs to distinct cerebellar stripes during goal-directed behavior. (2016)
  • Author(s): Tsutsumi S, Hidaka N, Isomura Y, Matsuzaki M, Sakimura K, Kano M, Kitamura K
  • Organizer: Neuroscience 2016
  • Place of Presentation: San Diego（USA）
  • Year and Date: 2016-11-15
  • Int'l Joint Research
• [Presentation] 2光子イメージングによる小脳回路研究:発達から運動機能まで (2016)
  • Author(s): 喜多村和郎
  • Organizer: 第63回中部生理学会
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県・岡崎市）
  • Year and Date: 2016-11-04
  • Invited
• [Presentation] 脳機能の2光子イメージング (2016)
  • Author(s): 喜多村和郎
  • Organizer: BioOpto Japan 2016
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-09-15
  • Invited
• [Presentation] Hyperacuity Bayesian technique to enhance temporal resolution of two-photon recording of complex spikes of cerebellar Purkinje cells. (2016)
  • Author(s): Hoang HT, Yamashita O, Tokuda IT, Kitamura K, Sato M, Kawato M, Toyama K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-07-22
  • Int'l Joint Research
• [Presentation] Cerebellar lobules receive spatiotemporally specific inputs from the cerebral cortex. (2016)
  • Author(s): Choo MJ, Hira R, Matsuzaki M, Kano M, Kitamura K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-07-22
  • Int'l Joint Research
• [Presentation] Functional organization of cerebellar modules during skilled behavior (2016)
  • Author(s): Kazuo Kitamura
  • Organizer: 第９３回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Optogenetic mapping of cerebra-cerebellar communication loop in mice (2016)
  • Author(s): Myeong Jeong Choo, Riichiro Hira, Masanori Matsuzaki, Masanobu Kano, Kazuo Kitamura
  • Organizer: 第９３回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Differential learning-related changes in climbing fiber inputs to cerebellar stripes (2016)
  • Author(s): Shinichiro Tsutsumi, Naoki Hidaka, Yoshikazu Isomura, Masanori Matsuzaki, Kenji Sakimura, Masanobu Kano, Kazuo Kitamura
  • Organizer: 第９３回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Rational design of ultrafast, high-affinity red calcium indicator for monitoring neuronal activity (2015)
  • Author(s): M. INOUE, A. TAKEUCHI, S.-I. HORIGANE, H. FUJII, S. KAMIJO, S. TAKEMOTO-KIMURA, M. OHKURA, K. GENGYO-ANDO, M. KANO, J. NAKAI, K. KITAMURA, H. BITO
  • Organizer: Neuroscience 2015
  • Place of Presentation: McCormick Place Convention Center (Chicago, USA)
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] In vivo光学イメージングと電気生理学の融合 (2015)
  • Author(s): 喜多村和郎
  • Organizer: 第３８回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-28
  • Invited
• [Presentation] Identification of complex spikes in two-photon recording of Purkinje cell calcium responses by machine learning and ROC analysis of the performance (2015)
  • Author(s): Huu Thien Hoang, Shinichiro Tsutsumi, Miki Hashizume, Okito Yamashita, Isao T. Tokuda, Masa-Aki Sato, Mitsuo Kawato, Masanobu Kano, Kazuo Kitamura, Keisuke Toyama
  • Organizer: 第３８回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Rational design of a novel high-affinity, ultrafast, red calcium indicator R-CaMP2 (2015)
  • Author(s): Masatoshi Inoue, Atsuya Takeuchi, Shin-Ichiro Horigane, Masamichi Ohkura, Keiko Gengyo-Ando, Hajime Fujii, Satoshi Kamijo, Sayaka Takemoto-Kimura, Masanobu Kano, Junichi Nakai, Kazuo Kitamura, Haruhiko Bito
  • Organizer: 第３８回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-28