Cell-type-specific patch-clamp recordings from deep brain of awake behaving mice
Project/Area Number |
15K14313
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Nagoya University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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Keywords | パッチクランプ / 大脳皮質 / マウス / 神経科学 / ドーパミン / 電気生理学 / 神経 |
Outline of Final Research Achievements |
To understand the neuronal mechanisms underlying animal behavior, it is essential to perform electrophysiological measurements of membrane potential dynamics of neurons in awake behaving animals with a high temporal resolution in a cell-type specific manner. In this study I verified technical feasibility of cell-type (or projection-target) specific patch-clamp recordings combined with optogenetics using task-performing mice. I successfully recorded membrane potentials of specific cell-types in multiple layers of primary somatosensory cortex of mice exhibiting spontaneous behavior or performing a simple learning task and addressed important questions on neuronal processing of whisker-related tactile information.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Near-infrared (NIR) up-conversion optogenetics.2015
Author(s)
Shoko Hososhima, Hideya Yuasa, Toru Ishizuka, Mohammad R. Hoque, Takayuki Yamashita, Akihiro Yamanaka, Eriko Sugano, Hiroshi Tomita, and Hiromu Yawo
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 16533-16533
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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