Development of measuring neural activity in a freely behaving animal

• Project/Area Number: 15K14315
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Kyoto University
• Principal Investigator: Watanabe Dai 京都大学, 医学研究科, 教授 (90303817)
• Co-Investigator(Renkei-kenkyūsha): Hamaguchi Kosuke 京都大学, 医学研究科, 講師 (50415270)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 電気生理学 / 細胞内記録 / 光学的計測 / 光遺伝学

Outline of Final Research Achievements

To measure electrical activity of the brain while a model animal is conducting cognitive-behavioral tasks is one of the most fundamental techniques for studying the brain function. To further understand how the neurons integrate synaptic inputs and generate action potentials, it is important to measure not only action potentials but also sub-threshold small potentials such as postsynaptic potentials. However, it is very difficult to measure sub-threshold potentials in a freely behaving animal, because the conventional electrophysiological devices for measuring sub-threshold potentials is very large and not suitable to the neural recording of behaving animals. In this study, we developed a small and light-weight electro-phsiological device which enables us to conduct intracellular recording to detect sub-threshold postsynaptic potentials.

Research Products

• [Journal Article] Contribution of propriospinal neurons to recovery of hand dexterity after corticospinal tract lesions in monkeys. (2017)
  • Author(s): Tohyama, T., Kinoshita, M., Kobayashi, K., Isa, K., Watanabe, D., Kobayashi, K., Liu, M., and Isa, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 114 Issue: 3 Pages: 604-609
  • DOI: 10.1073/pnas.1610787114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] mDia and ROCK mediate actin-dependent presynaptic remodeling regulating synaptic efficacy and anxiety. (2016)
  • Author(s): Deguchi Y, Harada M, Shinohara R, Lazarus M, Cherasse Y, Urade Y, Yamada D, Sekiguchi M, Watanabe D, Furuyashiki T, Narumiya S.
  • Journal Title: Cell Reports Volume: 17 Issue: 9 Pages: 2405-2417
  • DOI: 10.1016/j.celrep.2016.10.088
  • NAID: 120005868496
  • Peer Reviewed / Open Access
• [Journal Article] Calcium dysregulation contributes to neurodegeneration in FTLD patient iPSC-derived neurons (2016)
  • Author(s): Imamura, K., Sahara, N., Kanaan, N. M., Tsukita, K., Kondo, T., Kutoku, Y., Ohsawa, Y., Sunada, Y., Kawakami, K., Hotta, A., Yawata, S., Watanabe, D., Hasegawa, M., Trojanowski, J. Q., Lee, V. M.-Y., Suhara, T., Higuchi, M., and Inoue, H.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 34904-34904
  • DOI: 10.1038/srep34904
  • NAID: 120005850272
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transgenic songbirds with suppressed or enhanced activity of CREB transcription factor (2015)
  • Author(s): Abe, K., Matsui, S., and Watanabe, D.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 112 Issue: 24 Pages: 7599-7604
  • DOI: 10.1073/pnas.1413484112
  • NAID: 120005672630
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Critical role of the neural pathway from the intermediate medial mesopallium to the intermediate hyperpallium apicale in filial imprinting of domestic chicks (Gallus gallus domesticus). (2015)
  • Author(s): Aoki N, Yamaguchi S, Kitajima T, Takehara A, Katagiri-Nakagawa S, Matsui R, Watanabe D, Matsushima T, Homma KJ.
  • Journal Title: Neuroscience. Volume: 308 Pages: 115-124
  • DOI: 10.1016/j.neuroscience.2015.09.014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] CREB-mediated interplay of genes and environment in the postnatal song learning in songbirds (2016)
  • Author(s): Kentaro Abe, and Dai Watanabe
  • Organizer: Neuroscience 2016 (Society for Neuroscience)
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] Contribution of propriospinal neurons to recovery of hand dexterity after corticospinal tract lesions in monkeys (2016)
  • Author(s): Takamichi Tohyama, Masaharu Kinoshita, Kenta Kobayashi, Kaoru Isa, Dai Watanabe, Kazuto Kobayashi, Meigen Liu, and Tadashi Isa
  • Organizer: Neuroscience 2016 (Society for Neuroscience)
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] The Science of Birdsong (2016)
  • Author(s): Dai Watanabe
  • Organizer: Chanel：科学と音楽の出会い
  • Place of Presentation: 京都大学清風荘
  • Year and Date: 2016-09-24
  • Invited
• [Presentation] Genetic and neural circuit basis of learned vocalization (2016)
  • Author(s): 渡邉 大
  • Organizer: 新学術領域「適応回路シフト」国際シンポジウム
  • Place of Presentation: 同志社大学 室町キャンパス 寒梅館
  • Year and Date: 2016-03-03
  • Int'l Joint Research
• [Presentation] 高次脳機能を担う神経回路機構の理解に向けて (2016)
  • Author(s): 渡邉 大
  • Organizer: 平成28年度 京都大学脳神経外科教室同門会 Neuroscience Frontier
  • Place of Presentation: リーガロイヤルホテル大阪
  • Year and Date: 2016-02-13
  • Invited
• [Presentation] 音声スキル獲得・制御の神経回路機構 (2015)
  • Author(s): 渡邉 大
  • Organizer: 生理研研究会「大脳皮質の機能原理を探る」
  • Place of Presentation: 岡崎カンファレンスセンター
  • Year and Date: 2015-12-03
• [Remarks] Lab Home Page
  • URL: http://www.phy.med.kyoto-u.ac.jp/