| Project/Area Number |
15K14318
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | iPS 細胞 / 神経細胞 / 細胞死 / 細胞分化 / polβ / iPS細胞 / DNA修復酵素 / 遺伝子発現 / CRISPR/Cas9 / 大脳皮質 / 軸索 |
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| Outline of Final Research Achievements |
Our goal is to reveal characteristics of human neuronal differentiation and circuit formation using iPS cell technology. This study is aimed at making an experimental system to analyze the role of DNA polymerase β (polβ), by establishing the iPS cell line which lacks polβ. To establish the cell line, CRISPR/Cas9 system was utilized, and we could obtain the homozygote after drug selection. The obtained clone showed higher sensitivity than wild type clones. We further succeed in inducing neuronal stem cells and the neuronal cells.
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