Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Outline of Final Research Achievements |
In order to more precisely probe the selective synaptic connectivity occurring between neuron subtypes within local cortical circuits, we have developed a scanning electron microscope (SEM) observation that allows correlative light and electron microscopy of dendrites and axons originating from specific cortical cell subtypes. Using electron staining procedures and conductive section tapes suitable for SEM, we can now overlap fluorescent and electron microscopic images. Using this technique, we have found that the size of inhibitory synaptic junctions is correlated with the diameter of postsynaptic dendrites, and identified differences in synaptic input pattern across pyramidal cells subtypes.
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