Application of correlative light and scanning electron microscopy to pyramidal neuron circuits

• Project/Area Number: 15K14324
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: KAWAGUCHI Yasuo 生理学研究所, 基盤神経科学研究領域, 教授 (40169694)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 新皮質 / シナプス構造 / 走査型電子顕微鏡 / 光顕電顕相関 / 大脳皮質回路 / シナプス / 電子顕微鏡

Outline of Final Research Achievements

In order to more precisely probe the selective synaptic connectivity occurring between neuron subtypes within local cortical circuits, we have developed a scanning electron microscope (SEM) observation that allows correlative light and electron microscopy of dendrites and axons originating from specific cortical cell subtypes. Using electron staining procedures and conductive section tapes suitable for SEM, we can now overlap fluorescent and electron microscopic images. Using this technique, we have found that the size of inhibitory synaptic junctions is correlated with the diameter of postsynaptic dendrites, and identified differences in synaptic input pattern across pyramidal cells subtypes.

Research Products

• [Int'l Joint Research] マックスプランク研究所(ドイツ)
• [Int'l Joint Research] South Valley University(Egypt)
• [Int'l Joint Research] Institute of Neuroscience and Medicine(Germany)
• [Int'l Joint Research] South Valley University(Egypt)
• [Journal Article] A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure (2018)
  • Author(s): Kubota Y, Sohn J, Hatada S, Schurr M, StraehleJ, Gour A, Neujahr R, MikiT, Mikula S, Kawaguchi Y
  • Journal Title: Nat. Commun. Volume: 9 Issue: 1 Pages: 437-437
  • DOI: 10.1038/s41467-017-02768-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Segregated Excitatory?Inhibitory Recurrent Subnetworks in Layer 5 of the Rat Frontal Cortex (2017)
  • Author(s): Morishima Mieko、Kobayashi Kenta、Kato Shigeki、Kobayashi Kazuto、Kawaguchi Yasuo
  • Journal Title: Cerebral Cortex Volume: 27 Issue: 12 Pages: 5846-5857
  • DOI: 10.1093/cercor/bhx276
  • Peer Reviewed / Open Access
• [Journal Article] Pyramidal cell subtypes and their synaptic connections in layer 5 of rat frontal cortex (2017)
  • Author(s): Kawaguchi Y
  • Journal Title: Cereb Cortex Volume: 27 Issue: 12 Pages: 5755-5771
  • DOI: 10.1093/cercor/bhx252
  • Peer Reviewed / Open Access
• [Journal Article] 大脳皮質局所回路：古典的競合選択モデルの実験的検証と混合選択性細胞 (2017)
  • Author(s): 森田賢治, 川口泰雄
  • Journal Title: 生体の科学 Volume: 68 Pages: 43-47
• [Journal Article] Selective thalamic innervation of rat frontal cortical neurons. (2016)
  • Author(s): Shigematsu N, Ueta Y, Mohamed AA, Hatada S, Fukuda T, Kubota Y, Kawaguchi, Y
  • Journal Title: Cerebral Cortex Volume: 26 Issue: 6 Pages: 2689-2701
  • DOI: 10.1093/cercor/bhv124
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cortical divergent projections in mice originate from two sequentially generated, distinct populations of excitatory cortical neurons with different initial axonal outgrowth characteristics. (2015)
  • Author(s): Hatanaka, Y., Namikawa, T., Yamauchi, K., & Kawaguchi, Y.
  • Journal Title: Cereb. Cortex Volume: - Issue: 5 Pages: 2257-2270
  • DOI: 10.1093/cercor/bhv077
  • Peer Reviewed / Open Access
• [Journal Article] Functional effects of distinct innervation styles of pyramidal cells by fast spiking cortical interneurons (2015)
  • Author(s): Kubota Y, Kondo S, Nomura M, Hatada S, Yamaguchi N, Mohamed AA, Karube F, Luebke J, Kawaguchi Y
  • Journal Title: eLife Volume: 4
  • DOI: 10.7554/elife.07919
  • NAID: 120005997706
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Computing reward prediction error: an integrated account of cortical timing and basal-ganglia pathways for appetitive and aversive learning (2015)
  • Author(s): Morita K, Kawaguchi Y
  • Journal Title: Eur J Neurosci Volume: 42 Issue: 4 Pages: 2003-2021
  • DOI: 10.1111/ejn.12994
  • Peer Reviewed / Open Access
• [Journal Article] Temporal structure of neuronal activity among cortical neuron subtypes during slow oscillations in anesthetized rats (2015)
  • Author(s): Ushimaru M, Kawaguchi Y
  • Journal Title: J Neurosci Volume: 35 Issue: 34 Pages: 11988-12001
  • DOI: 10.1523/jneurosci.5074-14.2015
  • Peer Reviewed / Open Access
• [Presentation] Local recurrent subnetworks correlated with long-distance projections in frontal cortex (2018)
  • Author(s): Kawaguchi Y
  • Organizer: International symposium on Brain Information Dynamics 2018
  • Int'l Joint Research / Invited
• [Presentation] Ipsilateral and contralateral corticocortical projection-dependent subcircuits in layer 2/3 of rat frontal cortex (2018)
  • Author(s): Ueta Y, Kawaguchi Y
  • Organizer: 第95回日本生理学会大会
• [Presentation] Dynamics of thalamocortical activities during motor execution and learning (2018)
  • Author(s): Tanaka YH, Tanaka YR, Kondo M, Terada S, Kawaguchi Y, Matsuzaki M
  • Organizer: 第95回日本生理学会大会
• [Presentation] Synapse density and estimated number on various neurons of the rat frontal cortex (2017)
  • Author(s): Kubota Y,Sekigawa A,Mohamed AA,Kondo S,Karube F,Tanaka Y,Kawaguchi Y
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2017-03-28
  • Invited
• [Presentation] 前頭皮質における錐体細胞と抑制スタイルの多様性 (2017)
  • Author(s): 川口泰雄
  • Organizer: 第12回日本統合失調症学会
  • Place of Presentation: 米子コンベンションセンターBiG SHIiP（鳥取県米子市）
  • Year and Date: 2017-03-25
  • Invited
• [Presentation] ATUMtome 用の新開発テープと高解像画像用組織処理法 (2017)
  • Author(s): 窪田芳之, 孫在隣, 畑田小百合, 川口泰雄
  • Organizer: 第73回日本顕微鏡学会学術講演会
  • Invited
• [Presentation] Differential involvement of direct- and indirect-pathway spiny projection neurons in action valuation and update in the dorsomedial striatum (2017)
  • Author(s): Kimura M, Nonomura S, Nishizawa K, Kobayashi K, Sakai Y, Kawaguchi Y, Nambu A, Yamanaka K, Isomura Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] A novel tape for ATUMtome and high resolution electron microscopy (2017)
  • Author(s): Kubota Y, Sohn J, Hatada S, Kawaguchi Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] Labeling of neurons derived from intermediate neuronal progenitors in the mouse cerebral cortex (2017)
  • Author(s): Hatanaka Y, Hirata T, Kawaguchi Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] Cortical pyramidal cell-subtype dependent motor learning and oscillatory activities (2017)
  • Author(s): Otsuka T, Kawaguchi Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] Thalamocortical dynamics mediate learning and execution of self-initiated movement (2017)
  • Author(s): Tanaka YH, Tanaka YR, Hira R, Kondo M, Terada S, Kawaguchi Y, Matsuzaki M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Activity of direct- and indirect-pathway striatal projection neurons identified by optogenetics during adaptive choice task with probabilistic reward (2017)
  • Author(s): Nonomura S, Yamanaka K, Nishizawa K, Kobayashi K, Sakai Y, Kawaguchi Y, Nambu A, Isomura Y, Kimura M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Pyramidal cell subtype-dependent inhibitory innervations in layer 5 of the rat frontal cortex (2017)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] 前頭皮質のニューロン構成と標準回路 (2017)
  • Author(s): 川口泰雄
  • Organizer: 新学術領域研究「脳情報動態を規定する多領野連関と並列処理（脳情報動態）」領域公募説明会＆特別講演会「コネクトミクスからAIへ」
  • Invited
• [Presentation] Action-specific reinforcement and update by direct- and indirect- pathway striatal projection neurons (2017)
  • Author(s): Nonomura S, Nishizawa K, Kobayashi K, Sakai Y, Kawaguchi Y, Nambu A, Yamanaka K, Enomoto K, Isomura Y, Kimura M
  • Organizer: 47th annual meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] A conductive novel tape material and a new staining protocol for volume electron microscopy applications (2017)
  • Author(s): Kubota Y, Sohn J, Hatada S, Schurr M , Straehle J, Gour A. G, Neujahr R, Mikula S, Kawaguchi Y
  • Organizer: 47th annual meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Innervation differences of layer 5a and 5b Martinotti cells in frontal cortex (2017)
  • Author(s): Kawaguchi Y, Morishima M
  • Organizer: 47th annual meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] 前頭皮質ニューロンと再帰的結合の多様性 (2017)
  • Author(s): 川口泰雄
  • Organizer: 次世代脳プロジェクト冬のシンポジウム2017
  • Invited
• [Presentation] Layer 5 GABAergic Circuits in the Frontal Cortex (2016)
  • Author(s): Kawaguchi Y
  • Organizer: The 7th International Neural Microcircuit Conference- Recent advances in the analysis of cortical microcircuits -
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2016-12-09
  • Int'l Joint Research / Invited
• [Presentation] Oscillatory Activities in the Cortical Networks (2016)
  • Author(s): Otsuka T,Kawaguchi Y
  • Organizer: The 7th International Neural Microcircuit Conference- Recent advances in the analysis of cortical microcircuits -
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] Interaction between GABAergic cells and two types of target-specific pyramidal cells in layer 5 of the rat frontal cortex (2016)
  • Author(s): Morishima M,Kawaguchi Y
  • Organizer: The 7th International Neural Microcircuit Conference- Recent advances in the analysis of cortical microcircuits -
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] Conserved dimensions of dendritic trees in cortical pyramidal cell (2016)
  • Author(s): Mohamed AA,Yamaguchi N,Hatada S,Luebke J,Kawaguchi Y,Kubota Y
  • Organizer: The 7th International Neural Microcircuit Conference- Recent advances in the analysis of cortical microcircuits -
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] GABAergic/non-GABAergic synaptic inputs to striatal medium spiny neurons (2016)
  • Author(s): Kubota Y,Kawaguchi Y
  • Organizer: 46th annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego Convention Center （San Diego, USA）
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Activity of dopamine D2 receptor-expressing striatal neurons during decision-making task (2016)
  • Author(s): Nonomura S,Yamanaka K,Nishizawa K,Kobayashi K,Sakai Y,Kawaguchi Y,Nambu A,Isomura Y,Kimura M
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-22
• [Presentation] Interaction between GABAergic cells and two types of target-specific pyramidal cells in layer 5 of the rat frontal cortex (2016)
  • Author(s): Morishima M,Kawaguchi Y
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-22
• [Presentation] Corticocortical cell diversity in superficial layers of the rat frontal cortex (2016)
  • Author(s): Ueta Y,Hirai Y,Kawaguchi Y
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-21
• [Presentation] Distinct thalamocortical inputs mediate learning and execution of self-initiated movement (2016)
  • Author(s): Tanaka YH,Tanaka YR,Hira R,KondoM,Terada S,Kawaguchi Y,Matsuzaki M
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-21
• [Presentation] GABAergic inputs to spine of striatal medium spiny neuron (2016)
  • Author(s): Kubota Y,Kawaguchi Y
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
• [Presentation] Proper termination of migration for uppermost part of layers 2/3 neurons requires PlexinA2/A4-Semaphorin6A signaling in the mouse cerebral cortex (2016)
  • Author(s): Hatanaka Y,Kawasaki T,Kawaguchi Y,Hirata T
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
• [Presentation] An excitatory and inhibitory synapse density on various neurons in the rat frontal cortex (2016)
  • Author(s): Kubota Y,Sekigawa A,Mohamed AA,Kondo S,Karube F,Tanaka Y,Kawaguchi Y
  • Organizer: EMBO Dendrites 2016 meeting
  • Place of Presentation: Foundation for Research and Technology-Hellas（Creete, Greece）
  • Year and Date: 2016-06-20
  • Int'l Joint Research
• [Presentation] Pyramidal cell diversity and inhibition styles in the frontal cortex (2016)
  • Author(s): Kawaguchi Y, Morishima M
  • Organizer: International Symposium on Adaptive Circuit Shift 2016
  • Place of Presentation: 同志社大学（ 京都府京都市）
  • Year and Date: 2016-03-04
  • Int'l Joint Research / Invited
• [Presentation] Plexin A2/A4 - Semaphorin 6A signaling is involved in termination of migration for uppermost layer neurons in the mouse cerebral cortex (2016)
  • Author(s): Hatanaka Y, Kawasaki T, Hirata T, Kawaguchi Y
  • Organizer: International Symposium on Adaptive Circuit Shift 2016
  • Place of Presentation: 同志社大学（ 京都府京都市）
  • Year and Date: 2016-03-03
  • Int'l Joint Research
• [Presentation] Synapses from fast-spiking GABAergic neurons are tuned to specific postsynaptic target domains (2016)
  • Author(s): Kawaguchi Y, Kubota Y
  • Organizer: 新学術領域「 大脳新皮質構築」終了公開国際シンポジウム
  • Place of Presentation: 東京大学（ 東京都文京区）
  • Year and Date: 2016-02-12
  • Int'l Joint Research / Invited
• [Presentation] PlexinA2/A4 signaling is necessary for proper termination of migration for uppermost part of layers 2/3 neurons in the mouse cerebral cortex (2016)
  • Author(s): Hatanaka Y, Kawasaki T, Hirata T, Kawaguchi Y
  • Organizer: 新学術領域「 大脳新皮質構築」終了公開国際シンポジウム
  • Place of Presentation: 東京大学（ 東京都文京区）
  • Year and Date: 2016-02-12
  • Int'l Joint Research
• [Presentation] 大脳皮質パルブアルブミン細胞の歴史と謎 (2015)
  • Author(s): 川口泰雄
  • Organizer: 生理研研究会「 行動システム脳科学の新展開」
  • Place of Presentation: 生理学研究所（ 愛知県岡崎市）
  • Year and Date: 2015-12-04
• [Presentation] An excitatory and inhibitory synapse density on various nonpyramidal cells in the rat cerebral cortex (2015)
  • Author(s): Kubota Y, Sekigawa A, Hatada S, Kawaguchi Y
  • Organizer: The 2nd East-Asia Microscopy Conference
  • Place of Presentation: The Himeji Chamber of Commerce and Industry (Himeji, Hyogo)
  • Year and Date: 2015-11-25
  • Int'l Joint Research / Invited
• [Presentation] Temporal organization of pyramidal neuron output to the striatum (2015)
  • Author(s): Kawaguchi Y
  • Organizer: International Symposium on Prediction and Decision Making 2015
  • Place of Presentation: 東京大学（ 東京都文京区）
  • Year and Date: 2015-10-31
  • Int'l Joint Research
• [Presentation] Functional effects of distinct innervation styles of pyramidal cells by fast spiking cortical interneurons (2015)
  • Author(s): Kubota Y, Kondo S, Nomura M, Hatada S, Yamaguchi N, Mohamed AA, Karube F, Luebke J, Kawaguchi Y
  • Organizer: 45th annual meeting of the Society for Neuroscience
  • Place of Presentation: McCormick Place (Chicago, USA)
  • Year and Date: 2015-10-19
  • Int'l Joint Research
• [Presentation] Multi-compartmental models of corticopontine- and crossed corticostriatal neurons of rat frontal cortex (2015)
  • Author(s): Kondo M, Morishima M, Kawaguchi Y, Morita K
  • Organizer: 11th Bernstein Conference 2015
  • Place of Presentation: Neue Universitat (Heidelberg, Germany)
  • Year and Date: 2015-09-15
  • Int'l Joint Research
• [Presentation] Correlative light and electron microscopy of brain tissue using two-photon microscope and FIB/SEM (2015)
  • Author(s): Kubota Y, Villa KL, Berry KP, Subramanian J, Cha JW, So PT, Nedivi E, Kawaguchi Y
  • Organizer: Microscopy & Microanalysis 2015
  • Place of Presentation: Oregon Convention Center (Portland, USA)
  • Year and Date: 2015-08-03
  • Int'l Joint Research
• [Presentation] 徐波振動における前頭皮質ニューロンの発火時系列 (2015)
  • Author(s): 牛丸弥香、川口泰雄
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（ 兵庫県神戸市）
  • Year and Date: 2015-07-30
• [Presentation] Interaction between two types of pyramidal cells and GABAergic cells in the layer 5 of the frontal cortex (2015)
  • Author(s): 森島美絵子、川口泰雄
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（ 兵庫県神戸市）
  • Year and Date: 2015-07-30
• [Presentation] Conserved morphology of dendritic trees in cortical pyramidal cell (2015)
  • Author(s): Mohamed AA, Kubota Y, Kondo S, Yamaguchi N, Hatada S, Nomura M, Younes HA, Tanaka YR, Abdalla KEH, Luebke J, Kawaguchi Y
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（ 兵庫県神戸市）
  • Year and Date: 2015-07-29
• [Remarks] 生理学研究所大脳神経回路論研究部門
  • URL: http://www.nips.ac.jp/circuit/