Formation of discrete connectivity by the timing of spontaneous activity
| Project/Area Number |
15K14327
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Kyushu University (2017)
Institute of Physical and Chemical Research (2015-2016) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 神経回路形成 / 自発神経活動 / 嗅球 / カルシウムイメージング / 僧帽細胞 / 樹状突起 / 神経科学 / マウス |
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| Outline of Final Research Achievements |
In the olfactory system, discrete map formation in the olfactory bulb (OB) is essential for odor discrimination. It has been known that mitral cells prune all but one primary dendrite during develppment. Previously we found that dendrite pruning is perturbed by the over-expression of Kir2.1 or NMDAR knockout in mitral cells, and also regulated by spontaneous neuronal activity produced in the OB but not sensory-evoked activity. In this study, pharmacological experiments revealed that gap junctions and ionotropic glutamate receptors are required for the spontaneous network activity in the OB. This spontaneous activity was highly correlated among glomeruli at an early stage (P0-2); however, the pattern of the activity became decorrelated and glomerulus-specific just prior to the pruning, suggesting that this developmental transition promotes the formation of discrete connectivity in the OB.
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Report
(4 results)
Research Products
(31 results)
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[Presentation] 僧帽細胞樹状突起の刈り込みと接続特異性を制御する発達期の自発神経活動2016
Author(s)
Satoshi Fujimoto, Marcus N. Leiwe, Yuko Muroyama, Reiko Kobayakawa, Ko Kobayakawa, Tetsuichiro Saito, Takeshi Imai
Organizer
第39回日本神経科学大会
Place of Presentation
パシフィコ横浜（横浜市）
Year and Date
2016-07-20
Related Report
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[Presentation] SUPER-RESOLUTION MAPPING OF THE BRAIN USING SEEDB22016
Author(s)
Meng-Tsen Ke, Satoshi Fujimoto, Yasuhiro Nakai, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, Takeshi Imai
Organizer
Focus on Microscopy 2016
Place of Presentation
NTUH International Convention Center, Taipei, Taiwan
Year and Date
2016-03-20
Related Report
Int'l Joint Research
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[Presentation] Deep-tissue super-resolution imaging using an index optimized clearing agent2015
Author(s)
Meng-Tsen Ke, Satoshi Fujimoto, Yasuhiro Nakai, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, Takeshi Imai
Organizer
Cell Symposia: Engineering the Brain - Technologies for Neurobiological Applications
Place of Presentation
McCormick Place Convention Center, Chicago, IL, USA
Year and Date
2015-10-15
Related Report
Int'l Joint Research
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[Presentation] 嗅球神経回路の生後発達を制御する自発神経活動2015
Author(s)
Takeshi Imai, Satoshi Fujimoto, Marcus Leiwe, Yuko Muroyama, Tetsuichiro Saito
Organizer
第38回日本神経科学大会
Place of Presentation
神戸国際会議場（兵庫県神戸市）
Year and Date
2015-07-28
Related Report
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[Presentation] SeeDB2を用いた神経回路の深部超解像イメージング2015
Author(s)
Meng-Tsen Ke, Satoshi Fujimoto, Shuhei Yoshida, Rie Takayama, Tomoya Kitajima, Makoto Sato, Takeshi Imai
Organizer
第38回日本神経科学大会
Place of Presentation
神戸国際会議場（兵庫県神戸市）
Year and Date
2015-07-28
Related Report
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