| Project/Area Number |
15K14329
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
KINOSHITA Nagatoki 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (50391933)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAWAKI Atsushi 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (80251445)
SHIMOGORI Tomomi 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (30391981)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 蛍光プローブ / 神経回路 / 神経シナプス / 可視化技術 / 細胞間接着 |
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| Outline of Final Research Achievements |
Identification of the specific neuronal connectivity of neural circuits is crucial to reveal precise brain function that governs animal behavior. However, effectively labeling of specific neuronal connections in complicated networks is still quite difficult. Thus, I developed a novel genetically encoded fluorescent indicator for visualizing intercellular connections. This technique reconstituted robust GFP signal specifically at the interface between cell surfaces. Its signal intensity is much higher than that of similar technique previously reported. Application of this technique into the mouse nervous system demonstrated visualization of precise synaptic connectivity in vitro and in vivo. Moreover, I generated color variants of this probe molecule, which enable us to detect multiple convergent connections simultaneously. These results demonstrate that my system has high sensitivity and versatility, which will facilitate the analysis of complicated brain networks in vivo.
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