Why there are multiple synaptic adhesion molecules in single synapse? - Nanodomain hypothesis
Project/Area Number |
15K14330
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Hayashi Yasunori 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (90466037)
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Project Period (FY) |
2015-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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Keywords | シナプス / 細胞接着因子 / 超高解像度顕微鏡 |
Outline of Final Research Achievements |
Multiple synaptic adhesion factors have been identified so far but why they coexist in one type of synapse is not clear. We hypothesized that each of them forms different nano-domain. In order to test this, we carried out super-resolution imaging of immunostaining signal of neuroligin and EphB. We could observe distribution of a postsynaptic protein Homer, as expected from its postsynaptic function. On the other hand, for neuroligin and EphB, the signal was too weak to convincingly conclude anything. Both are membrane protein and we speculated that low copy number of proteins. Therefore, we are currently generating epitope-tagged constructs.
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Report
(2 results)
Research Products
(6 results)
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[Journal Article] A Temporary Gating of Actin Remodeling during Synaptic Plasticity Consists of the Interplay between the Kinase and Structural Functions of CaMKII.2015
Author(s)
Kim, K., Lakhanpal, G., Lu, HE., Khan, M., Suzuki, A., Kato-Hayashi, M., Narayanan, R., Luyben, TT., Matsuda, T., Nagai, T., Blanpied, TA., Hayashi, Y. and Okamoto K.
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Journal Title
Neuron
Volume: 87
Issue: 4
Pages: 813-826
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice.2015
Author(s)
Sakaguchi M, Kim K, Yu LM, Hashikawa Y, Sekine Y, Okumura Y, Kawano M, Hayashi M, Kumar D, Boyden ES, McHugh TJ, Hayashi Y.
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Journal Title
PLoS One.
Volume: 10
Issue: 6
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A top-down cortical circuit for accurate sensory perception2015
Author(s)
Manita S, Suzuki T, Homma C, Matsumoto T, Odagawa M, Yamada K, Ota K, Matsubara C, Inutsuka A, Sato M, Ohkura M, Yamanaka A, Yanagawa Y, Nakai J, Hayashi Y, Larkum ME, Murayama M
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Journal Title
Neuron
Volume: 86(5)
Issue: 5
Pages: 1304-16
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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