New infection directivity by modification of capsid proteins of adeno-associated virus.
| Project/Area Number |
15K14333
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 神経科学 / 神経回路網 / アデノ随伴ウイルス / キャプシド / 特異的感染 |
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| Outline of Final Research Achievements |
We develop new technologies that allow neural cell type-specific and neural pathway-specific gene transduction with viral vector alone by modification of AAV capsid proteins. The strategy is simple, and we pursued research based on the following two policies. (i) Removal of nonspecific infectivity: it was confirmed by cultured cells that infectivity was dramatically decreased by replacing various amino acid sequences prescribing extensive infection directivity. Subsequently, verification is underway with primary cultured neurons and adult mice. (ii) Granting new infectivity: we aim to acquire new infectivity by adding / inserting specific sequence to capsid protein. Candidate specific sequences were prepared and purified. We are proceeding with verification in adult mouse.
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Report
(3 results)
Research Products
(54 results)
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[Journal Article] ScaleS: an optical clearing palette for biological imaging.2016
Author(s)
Hama H, Hioki H, Namiki K, Hoshida T, Kurokawa H, Ishidate F, Kaneko T, Akagi T, Saito T, Saido T, *Miyawaki A.
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Journal Title
Nature Neuroscience
Volume: 18
Issue: 10
Pages: 1518-1529
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Dorsal Horn Circuits for Persistent Mechanical Pain.2015
Author(s)
Peirs C, Williams SP, Zhao X, Walsh CE, Gedeon JY, Cagle NE, Goldring AC, Hioki H, Liu Z, Marell PS, *Seal RP.
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Journal Title
Neuron
Volume: 87
Issue: 4
Pages: 797-812
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Dorsal and ventral parts of the rat thalamic submedial nucleus provide two independent inputs to different areas of orbital cortex.2017
Author(s)
Kuramoto E, Iwai H, Yamanaka A, Seki H, Ohno S, Sendo R, Koyanagi K, Toyodome S, Furuta T, Hioki H, Goto T.
Organizer
第122回日本解剖学会総会・全国学術集会
Place of Presentation
長崎
Year and Date
2017-03-28
Related Report
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[Presentation] A morphological analysis of thalamocortical projections arising from the rat mediodorsal nucleus: A single neuron-tracing study using viral vectors.2015
Author(s)
*Kuramoto E, Pan S, Furuta T, Hioki H, Iwai H, Yamanaka A, Ohno S, Goto T, Kaneko T.
Organizer
The 45th Society for Neuroscience Annual Meeting (Neuroscience 2015)
Place of Presentation
Chicago, IL, USA
Year and Date
2015-10-20
Related Report
Int'l Joint Research
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