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Elucidation of the molecular basis of neural stem cell niche using synthetic polymer arrays

Research Project

Project/Area Number 15K14346
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TAGA Tetsuya  東京医科歯科大学, 難治疾患研究所, 教授 (40192629)

Research Collaborator BRADLEY Mark  University of Edinburgh, School of Chemistry, Professor
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords神経幹細胞 / ニッチ / 自己複製 / ポリマー / 神経科学 / 発生・分化 / シグナル伝達
Outline of Final Research Achievements

Neural stem cells (NSCs) require a specific niche for their maintenance. However, its molecular basis is poorly understood. Although conventional approaches have been extensively done, the niche condition is still required to be studied. To solve this problem, we have established a polymer-based microarray system for screening NSC niche-mimicry. NSCs isolated from E14.5 mouse embryos were seeded on a glass slide on which 376 kinds of polymers were spotted. They attached strongly to several kinds of polymers. Among them, one acrylate-based polymer called PA518 maintained self-renewal capacity of NSCs, in spite of the differentiation-inducing FGF2-deficient condition. A control polymer, PA417, without such activity was also selected. Six proteins with different molecular weights were identified by SDS-PAGE of the proteins specifically bound to PA518. These achievements may contribute to the understanding of NSC niche by further study including mass spectrometric analysis.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (32 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 2 results,  Open Access: 3 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (2 results) Remarks (5 results)

  • [Int'l Joint Research] School of Chemistry, Univ. of Edinburgh(United Kingdom)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] School of Chemistry, Univ. of Edinburgh(United Kingdom)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] School of Chemistry, Univ. of Edinburgh(United Kingdom)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Maintenance of hematopoietic stem and progenitor cells in fetal intra-aortic hematopoietic clusters by the Sox17-Notch1-Hes1 axis.2018

    • Author(s)
      Saito K, Nobuhisa I, Harada K, Takahashi S, Anani M, Lickert H, Kanai-Azuma M, Kanai Y, Taga T.
    • Journal Title

      Exp Cell Res

      Volume: 365 Issue: 1 Pages: 145-155

    • DOI

      10.1016/j.yexcr.2018.02.014

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gfap and Osmr regulation by BRG1 and STAT3 via interchromosomal gene clustering in astrocytes2018

    • Author(s)
      Ito Kenji、Noguchi Azumi、Uosaki Yuichi、Taga Testuya、Arakawa Hirokazu、Takizawa Takumi
    • Journal Title

      Molecular Biology of the Cell

      Volume: 29 Issue: 2 Pages: 209-219

    • DOI

      10.1091/mbc.e17-05-0271

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thrombopoietin contributes to the formation and the maintenance of hematopoietic progenitor-containing cell clusters in the aorta-gonad-mesonephros region.2017

    • Author(s)
      Harada K, Nobuhisa I, Anani M, Saito K, and Taga T
    • Journal Title

      Cytokine

      Volume: 95 Pages: 35-42

    • DOI

      10.1016/j.cyto.2017.02.012

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Enhancement of 5-aminolevulinic acid-based fluorescence detection of side population-defined glioma stem cells by iron chelation2017

    • Author(s)
      Wang W, Tabu K, Hagiya Y, Sugiyama Y, Kokubu Y, Murota Y, Ogura SI and Taga T
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 42070-42070

    • DOI

      10.1038/srep42070

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A synthetic polymer scaffold reveals the self-maintenance strategies of rat glioma stem cells by organization of the advantageous niche.2016

    • Author(s)
      Tabu K, Muramatsu N, Mangani C, Wu M, Zhang R, Kimura T, Terashima K, Bizen N, Kimura R, Wang W, Murota Y, Kokubu Y, Nobuhisa I, Kagawa T, Kitabayashi I, Bradley M, Taga T.
    • Journal Title

      Stem Cells

      Volume: 34 Issue: 5 Pages: 1151-1162

    • DOI

      10.1002/stem.2299

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An increase of GFAP-positive astrocytes in histone demethylase GASC1/KDM4C/JMJD2C hypomorphic mutant mice.2016

    • Author(s)
      Sudo G, Kagawa T, Kokubu Y, Inazawa J, Taga T.
    • Journal Title

      Genes to Cells

      Volume: 21 Issue: 3 Pages: 218-225

    • DOI

      10.1111/gtc.12331

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 神経膠腫癌幹細胞の利己的生存戦略による癌の進展2017

    • Author(s)
      田賀哲也、椨康一
    • Organizer
      第38回日本 炎症・再生医学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Self-expanding strategies of glioma stem cells that involves macrophages to adapt to iron-deprivation stress2017

    • Author(s)
      Tabu K, Wang W, Murota Y and Taga T
    • Organizer
      The 76th Annual Meeting of the Japanese Cancer Association
    • Related Report
      2017 Annual Research Report
  • [Presentation] Establishment of glioma mouse model by transducing oncogenic H-RasV12 gene into the p53 deficient astrocytes2017

    • Author(s)
      Murota Y, Wenqing GY, Tabu K, and Taga T
    • Organizer
      The 76th Annual Meeting of the Japanese Cancer Association
    • Related Report
      2017 Annual Research Report
  • [Presentation] 宿主単球・マクロファージの誘導によるグリオーマ幹細胞の鉄欠乏ストレスへの適応:ニッチ自己構築機構の解明2017

    • Author(s)
      椨 康一、巽 瑠璃子、田賀 哲也
    • Organizer
      Consortium of Biological Sciences 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] グリオーマ幹細胞による宿主マクロファージ制御におけるオートスキジス様細胞死の役割2017

    • Author(s)
      松永 浩明、椨 康一、田賀 哲也
    • Organizer
      Consortium of Biological Sciences 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 膵臓癌幹細胞ニッチを擬態する合成ポリマーのスクリーニング2017

    • Author(s)
      室田 吉貴、Sara Schmidt、椨 康一、伊藤 浩光、田中 真二、Mark Bradley、田賀 哲也
    • Organizer
      Consortium of Biological Sciences 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Screening for human pancreatic cancer stem cell niche mimicry by using synthetic polymer microarrays2017

    • Author(s)
      Murota Y, Schmidt S, Tabu K, Ito H, Tanaka S, Bradley M and Taga T
    • Organizer
      The 15th Stem Cell Research Symposium
    • Related Report
      2017 Annual Research Report
  • [Presentation] The mechanism of maintaining the undifferentiated state of hematopoietic stem cell by Sox17 and TET family members.2017

    • Author(s)
      Saito K, Nobuhisa I, and Taga T
    • Organizer
      The 40th Annual Meeting of the Molecular Biology Society of Japan
    • Related Report
      2017 Annual Research Report
  • [Presentation] Role of adhesion molecules induced by a transcription factor Sox17 in intra-aortic hematopoietic cell cluster formation in midgestation mouse embryos.2017

    • Author(s)
      Nobuhisa I, Takahashi S, Saito K, and Taga T
    • Organizer
      The 46th Annual Meeting of The Japanese Society for Immunology
    • Related Report
      2017 Annual Research Report
  • [Presentation] Astroglial development is regulated by DNA and histone methylation: from molecular basis to behavioral abnormalities in gene-manipulated mice.2016

    • Author(s)
      Tetsushi Kagawa, Yuhei Yamaguchi, Genki Sudo, Yasuhiro Kokubu, Satoko Hattori, Keizo Takao, Tsuyoshi Miyakawa, Johji Inazawa, Tetsuya Taga
    • Organizer
      Cold Spring Harbor Asia meeting on Novel Insights into Glia Function & Dysfunction December 5-9, 2016
    • Place of Presentation
      Suzhou Dushu Lake Conference Center (Suzhou, China)
    • Year and Date
      2016-12-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Adaptive response of C6 glioma stem cells to iron deprivation through macrophage induction2016

    • Author(s)
      Tabu K, Wang W, Murota Y and Taga T
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Enhancement of 5-aminolevulinic acid-based fluorescence detection of glioma stem cells by chelating iron2016

    • Author(s)
      Wang W, Tabu K, Hagiya Y, Murota Y, Ogura SI and Taga T
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Adaptive response of rat C6 glioma stem cells to iron deprivation by which the development of tumor infiltrating macrophages is induced2016

    • Author(s)
      Tabu K, Kokubu Y, Muramatsu N, Nomoto S, Wang W and Taga T
    • Organizer
      第14回幹細胞シンポジウム
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2016-05-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Resistance of glioma stem cells to 5-aminolevulinic acid (ALA)-based detection due to enhanced metabolic conversion of protoporphyrin IX2016

    • Author(s)
      Wang W, Tabu K, Sugiyama Y, Hagiya Y, Ogura SI and Taga T
    • Organizer
      第14回幹細胞シンポジウム
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2016-05-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Synthetic polymer-based approach revealed an adaptive capacity of glioma stem cells by inducing tumor-infiltrating and iron-accumulating macrophages2015

    • Author(s)
      Kouichi Tabu, Nozomi Muramatsu, Tetsuya Taga
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県、神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Characterization of C6 glioma cancer stem cells and their niche2015

    • Author(s)
      Taga T and Tabu K
    • Organizer
      Seoul National University, Cancer Research Institute (SNUCRI) Annual Symposium 2015 ‘Innovative Approaches to Explore Novel Druggable Targets’
    • Place of Presentation
      Hwasun Kumho Resort (Hwasun, Korea)
    • Year and Date
      2015-04-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] がん幹細胞によるがん進展メカニズム(次世代がん治療)2017

    • Author(s)
      椨康一、田賀哲也
    • Total Pages
      386
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2017 Annual Research Report
  • [Book] 合成ポリマーを用いた癌幹細胞ニッチの特性解明 (バイオマテリアル -生体材料-)2017

    • Author(s)
      椨康一、室田吉貴、田賀哲也
    • Total Pages
      82
    • Publisher
      日本バイオマテリアル学会
    • Related Report
      2016 Research-status Report
  • [Remarks] 東京医科歯科大学 難治疾患研究所 幹細胞制御分野 研究内容

    • URL

      http://www.tmd.ac.jp/mri/scr/subjects/index.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report 2015 Research-status Report
  • [Remarks] Dept Stem Cell Regulation, MRI, TMDU: Res Subject

    • URL

      http://www.tmd.ac.jp/mri/scr/english/subjects/index.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report 2015 Research-status Report
  • [Remarks] 研究情報データベース 基本情報 田賀哲也

    • URL

      http://reins.tmd.ac.jp/html/100007289_ja.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report 2015 Research-status Report
  • [Remarks] Res. Information DB: Personnel Info.: TAGA Tetsuya

    • URL

      http://reins.tmd.ac.jp/html/100007289_en.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report 2015 Research-status Report
  • [Remarks] プレスリリース:癌再発に深く関わる癌幹細胞が診断薬5-ALAによる検出を免れる特性を発見

    • URL

      http://www.tmd.ac.jp/archive-tmdu/kouhou/20170207_1.pdf

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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