Elucidation of the molecular basis of neural stem cell niche using synthetic polymer arrays
| Project/Area Number |
15K14346
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TAGA Tetsuya 東京医科歯科大学, 難治疾患研究所, 教授 (40192629)
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| Research Collaborator |
BRADLEY Mark University of Edinburgh, School of Chemistry, Professor
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 神経幹細胞 / ニッチ / 自己複製 / ポリマー / 神経科学 / 発生・分化 / シグナル伝達 |
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| Outline of Final Research Achievements |
Neural stem cells (NSCs) require a specific niche for their maintenance. However, its molecular basis is poorly understood. Although conventional approaches have been extensively done, the niche condition is still required to be studied. To solve this problem, we have established a polymer-based microarray system for screening NSC niche-mimicry. NSCs isolated from E14.5 mouse embryos were seeded on a glass slide on which 376 kinds of polymers were spotted. They attached strongly to several kinds of polymers. Among them, one acrylate-based polymer called PA518 maintained self-renewal capacity of NSCs, in spite of the differentiation-inducing FGF2-deficient condition. A control polymer, PA417, without such activity was also selected. Six proteins with different molecular weights were identified by SDS-PAGE of the proteins specifically bound to PA518. These achievements may contribute to the understanding of NSC niche by further study including mass spectrometric analysis.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] A synthetic polymer scaffold reveals the self-maintenance strategies of rat glioma stem cells by organization of the advantageous niche.2016
Author(s)
Tabu K, Muramatsu N, Mangani C, Wu M, Zhang R, Kimura T, Terashima K, Bizen N, Kimura R, Wang W, Murota Y, Kokubu Y, Nobuhisa I, Kagawa T, Kitabayashi I, Bradley M, Taga T.
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Journal Title
Stem Cells
Volume: 34
Issue: 5
Pages: 1151-1162
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Astroglial development is regulated by DNA and histone methylation: from molecular basis to behavioral abnormalities in gene-manipulated mice.2016
Author(s)
Tetsushi Kagawa, Yuhei Yamaguchi, Genki Sudo, Yasuhiro Kokubu, Satoko Hattori, Keizo Takao, Tsuyoshi Miyakawa, Johji Inazawa, Tetsuya Taga
Organizer
Cold Spring Harbor Asia meeting on Novel Insights into Glia Function & Dysfunction December 5-9, 2016
Place of Presentation
Suzhou Dushu Lake Conference Center (Suzhou, China)
Year and Date
2016-12-05
Related Report
Int'l Joint Research / Invited
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