| Project/Area Number |
15K14351
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Neurochemistry/Neuropharmacology
|
|---|
| Research Institution | Ehime University |
|---|
Principal Investigator |
Tanaka Junya 愛媛大学, 医学系研究科, 教授 (70217040)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | microglia / circadian / sleep / synapse / noradrenaline / EEG / phagocytosis / eat-me signal / マイクログリア / シナプス / 貪食 / 睡眠覚醒リズム / 入眠覚醒リズム / グルタミン酸 / ノルアドレナリン |
|---|
| Outline of Final Research Achievements |
① Microglial cells become activated during sleeping time (light period; Zeitgeber Time (ZT0-12), while actively phagocytizing synapses. Around the waking time (dark period; ZT12-24), microglial phagocytic activity is suppressed. Consequently, amounts of synapses reduced during the light period as revealed by Western blotting.② Circadian changes in the concentration of noradrenaline caused the changes in the activity of microglial cells. Higher concentration of noradrenaline was correlated with suppressed microglial activity.③ It is likely that elimination of synapses by microglial cells causes asleep and maintain the sleep.④ Microglial cells eliminate only synapses with immunoreactivity of C3 or MFG-E8, suggesting the involvement of microglial cells during asleep in memory consolidation.
|
