New technique to sort cells expressing a gene of interest

• Project/Area Number: 15K14361
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Ema Masatsugu 滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞生物学 / 多能性幹細胞 / 遺伝子発現 / 胚性幹細胞

Outline of Final Research Achievements

A technique to highlight cells expressing gene of interest is a critical for hematopoietic stem cell research, and so on. Nano-gold was conjugated to a oligo complimentary to a gene of interest and used to know Rex1 positive cells in mouse ES cells.After purification with FACS, we have performed RT-qPCR methods and found that this techqnique is a poewerful one to sort a population of cells expressing a gene of interest.

Research Products

• [Journal Article] Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells. (2016)
  • Author(s): Khoa le TP, Azami T, Tsukiyama T, Matsushita J, Tsukiyama-Fujii S, Takahashi S, Ema M.
  • Journal Title: PLoS ONE Volume: 11 Issue: 3 Pages: e0150715-e0150715
  • DOI: 10.1371/journal.pone.0150715
  • NAID: 120007129540
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Functional crosstalk between Myc and PI3K signaling for supporting unlimited self-renewal of embryonic stem cells (2015)
  • Author(s): Hishida T, Nakachi Y, Mizuno Y, Katano M, Okazaki Y, Ema M, Takahashi S, Hirasaki M, Suzuki A, Ueda A, Nishimoto M, Hishida-Nozaki Y, Vazquez-Ferrer E, Sancho-Martinez I, Izpisua Belmonte JC, Okuda A.
  • Journal Title: STEM CELLS Volume: 33 Issue: 3 Pages: 713-725
  • DOI: 10.1002/stem.1893
  • Peer Reviewed / Open Access
• [Journal Article] Forced expression of Nanog or Esrrb preserves the ESC status in the absence of nucleostemin expression (2015)
  • Author(s): Katano M, Ema M, Nakachi Y, Mizuno Y, Hirasaki M, Suzuki A, Ueda A, Nishimoto M, Takahashi S, Okazaki, Okuda A.
  • Journal Title: STEM CELLS Volume: 33 Issue: 4 Pages: 1089-1101
  • DOI: 10.1002/stem.1918
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Remarks] 研究室ホームページ
  • URL: http://lab.rcals.jp