| Project/Area Number |
15K14361
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
Ema Masatsugu 滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞生物学 / 多能性幹細胞 / 遺伝子発現 / 胚性幹細胞 |
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| Outline of Final Research Achievements |
A technique to highlight cells expressing gene of interest is a critical for hematopoietic stem cell research, and so on. Nano-gold was conjugated to a oligo complimentary to a gene of interest and used to know Rex1 positive cells in mouse ES cells.After purification with FACS, we have performed RT-qPCR methods and found that this techqnique is a poewerful one to sort a population of cells expressing a gene of interest.
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