• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Genetic engineering of primordial germ cells for germline modification

Research Project

Project/Area Number 15K14362
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionKyoto University

Principal Investigator

Shinohara Takashi  京都大学, 医学研究科, 教授 (30322770)

Co-Investigator(Renkei-kenkyūsha) MORIMOTO Hiroko  京都大学, 大学院医学研究科, 助教 (90540097)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords始原生殖細胞 / 精子形成 / 卵子形成 / Kit
Outline of Final Research Achievements

Embryonic stem cells are widely used for germline modification. However, this technique is mostly limited to mice and such technology has not been applied to other animal species. To overcome this problem, we focused on primordial germ cells (PGCs). Because PGCs can proliferate very actively in vivo and can differentiate into sperm and oocytes, they represent an attractive target for germline modification. Therefore, we focused on the development of a long-term culture system for mouse PGCs. Although we were not able to find any positive results using gene transfection, 12 small chemicals induced proliferation of PGCs by screening > 3,000 chemical compounds. Of these, 5 chemicals were able to support PGCs for at least 2 months. We transplanted the cultured PGCs into the seminiferous tubules of infertile mice. However, we were not able to find any germ cell colonies. These results suggested that the candidate chemicals are not suitable for sustaining functionally active PGCs.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2018 2017 2016 2015

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 4 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Culture and transplantation of spermatogonial stem cells.2018

    • Author(s)
      Takashima, S. and Shinohara, T.
    • Journal Title

      Stem Cell Res.

      Volume: 29 Pages: 46-55

    • DOI

      10.1016/j.scr.2018.03.006

    • NAID

      120006498245

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transfer of a mouse artificial chromosome into spermatogonial stem cells genetates transchromosomic mice,2017

    • Author(s)
      Shinohara T, Kazuki K, Ogonuki N, Morimoto H, Matoba S, Hiramatsu K, Honma K, Suzuki T, Hara T, Ogura A, Oshimura M, Kanatsu-Shinohara M, Kazuki Y.
    • Journal Title

      Stem Cell Reports.

      Volume: 9(4) Issue: 4 Pages: 1180-1191

    • DOI

      10.1016/j.stemcr.2017.08.012

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nonrandom contribution of left and right testes to germline transmission from mouse spermatogonial stem cells2017

    • Author(s)
      Mito Kanatsu-Shinohara, Honda Naoki, Takashi Shinohara
    • Journal Title

      Biology of Reproduction

      Volume: 97(6) Issue: 6 Pages: 902-910

    • DOI

      10.1093/biolre/iox141

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enrichment of Mouse Spermatogonial Stem Cells by the Stem Cell Dye CDy1.2015

    • Author(s)
      Kanatsu-Shinohara M, Morimoto H, Shinohara T.
    • Journal Title

      Biology of reproduction

      Volume: 94 (1) 13 Issue: 1 Pages: 1-10

    • DOI

      10.1095/biolreprod.115.135707

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Pluripotent cell derivation from male germline cells by suppression of <i>Dmrt1</i> and <i>Trp53</i>2015

    • Author(s)
      Tanaka, T., Kanatsu-Shinohara, M., Hirose, M., Ogura, A., Shinohara, T.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 61 Issue: 5 Pages: 473-484

    • DOI

      10.1262/jrd.2015-059

    • NAID

      130005104118

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The CDKN1B-RB1-E2F1 pathway protects mouse spermatogonial stem cells from genomic damage2015

    • Author(s)
      Tanaka, T., Kanatsu-Shinohara, M., Shinohara, T.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 61 Issue: 4 Pages: 305-316

    • DOI

      10.1262/jrd.2015-027

    • NAID

      130005094647

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ROS-Generating Oxidase Nox3 Regulates the Self-Renewal of Mouse Spermatogonial Stem Cells2015

    • Author(s)
      Morimoto H, Kanatsu-Shinohara M, Shinohara T.
    • Journal Title

      Biology of reproduction

      Volume: 92 (6) 147 Issue: 6 Pages: 1-10

    • DOI

      10.1095/biolreprod.114.127647

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal.2015

    • Author(s)
      Takashima S, Kanatsu-Shinohara M, Tanaka T, Morimoto H, Inoue K, Ogonuki N, Jijiwa M, Takahashi M, Ogura A, Shinohara T.
    • Journal Title

      Stem Cell Reports.

      Volume: 4 Issue: 3 Pages: 489-502

    • DOI

      10.1016/j.stemcr.2015.01.010

    • NAID

      120005540400

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Fertility and spermatogonial stem cells2017

    • Author(s)
      Takashi Shinohara
    • Organizer
      American Society of Andrology 2017 42nd Annual Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 精子幹細胞の遺伝情報伝達パターン2017

    • Author(s)
      篠原隆司
    • Organizer
      放射線影響学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Myc-mediated glycolysis enhances spermatogonial stem cell self-renewal.2017

    • Author(s)
      Takashi Shinohara
    • Organizer
      第15回 幹細胞シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Non-random germline transmission of mouse spermatogonial stem cells2016

    • Author(s)
      Takashi Shinohara
    • Organizer
      internatinal symposium on epigenome dynamics and regulation in germ cells
    • Place of Presentation
      京都大学
    • Year and Date
      2016-02-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 雄性生殖細胞またはセルトリ細胞にポリヌクレオチドを導入する方法2017

    • Inventor(s)
      篠原隆司、渡邉哲史
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi