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A life design model using mutator mice

Research Project

Project/Area Number 15K14368
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionOsaka University

Principal Investigator

YAGI Takeshi  大阪大学, 生命機能研究科, 教授 (10241241)

Co-Investigator(Renkei-kenkyūsha) UCHIMURA Arikuni  大阪大学, 大学院生命機能研究科, 助教 (20513063)
Research Collaborator HIGUCHI Mayumi  
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords疾患モデル / マウス / 突然変異 / 量的形質 / DNA複製 / 生殖細胞 / ゲノム / 塩基配列 / 遺伝学 / 進化 / 多様性 / 生活習慣病
Outline of Final Research Achievements

In order to generate animal models for human diseases, we established mutator mouse strains which have high DNA replication error rates. We analyzed germline mutation rates and the long-term effects of mutation accumulation on phenotype in more than 20 generations of wild-type mice and mutator mice. We were successful to estimate the base-substitution mutation rate in C57BL/6 laboratory mice. The mutation rate in mutator mice was 17 times that in wild-type mice. As a result, we obtained phenotypes of human diseases from mutator mice.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (3 results)

  • [Journal Article] Clustered protocadherins are required for building functional neural circuits.2017

    • Author(s)
      Hasegawa S, Kobayashi H, Kumagai M, Nishimaru H, Tarusawa E, Kanda H, Kaneko R, Sanbo M, Yoshimura Y, Hirabayashi M, Hirabayashi T, Yagi T
    • Journal Title

      Front Mol Neurosci

      Volume: 印刷中 Pages: 114-114

    • DOI

      10.3389/fnmol.2017.00114

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct and Cooperative Functions for the Protocadherin-alfa, -beta and -ganma Clusters in Neuronal Survival and Axon Targeting.2016

    • Author(s)
      Hasegawa S, Kumagai M, Hagiwara M, Nishimaru H, Hirano K, Kaneko R, Okayama A, Hirayama T, Sanbo M, Hirabayashi M, Watanabe M, Hirabayashi T, Yagi T
    • Journal Title

      Front Mol Neurosci,

      Volume: 9 Pages: 155-155

    • DOI

      10.3389/fnmol.2016.00155

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of high reciprocal connectivity between clonal cortical neurons is regulated by the Dnmt3b DNA methyltransferase and clustered protocadherins2016

    • Author(s)
      Tarusawa E., Sanbo M., Okayama A., Miyashita T., Kitsukawa T., Hirayama T., Hirabayashi T., Hasegawa S., Kaneko R., Toyoda S., Kobayashi T., Kato-Itoh M., Nakauchi H., Hirabayashi M., Yagi T., Yoshimura Y.
    • Journal Title

      BMC Biology

      Volume: 14 Issue: 1 Pages: 103-103

    • DOI

      10.1186/s12915-016-0326-6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice2015

    • Author(s)
      Arikuni Uchimura, Mayumi Higuchi, Yohei Minakuchi, Mizuki Ohno, Atsushi Toyoda, Asao Fujiyama, Ikuo Miura, Shigeharu Wakana, Jo Nishino, Takeshi Yagi
    • Journal Title

      Genome Research

      Volume: 25 Issue: 8 Pages: 1125-1134

    • DOI

      10.1101/gr.186148.114

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 生殖系列の変異率と変異蓄積マウス系統2015

    • Author(s)
      内村有邦
    • Journal Title

      実験医学

      Volume: 33 Pages: 3123-3126

    • Related Report
      2015 Research-status Report
  • [Journal Article] 集団遺伝学から考える脳の進化2015

    • Author(s)
      内村有邦、八木健
    • Journal Title

      Clinical Neuroscience

      Volume: 33 Pages: 909-911

    • Related Report
      2015 Research-status Report
  • [Presentation] Mutation accumulation experiment with laboratory mice2017

    • Author(s)
      Uchimura A,Yagi T
    • Organizer
      International Symposium "The start of New Genomics"
    • Place of Presentation
      東京大学 伊藤国際学術研究センター(東京都文京区)
    • Year and Date
      2017-01-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウスを用いた実験室内進化モデルは、ヒトも含めた哺乳類の進化研究に新たな方法論を提供する2016

    • Author(s)
      内村有邦、樋口真弓、水口洋平、豊田敦、藤山秋佐夫、若菜茂晴、西野穣、八木健
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 実験用マウスの生殖系列の突然変異率とその後世代への影響2016

    • Author(s)
      内村有邦、樋口真弓、水口洋平、豊田敦、藤山秋佐夫、若菜茂晴、西野穣、八木健
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      ミューザ川崎シンフォニーホール(神奈川県川崎市)
    • Year and Date
      2016-05-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] Genetic codes for complex neural networks in the brain2015

    • Author(s)
      八木 健
    • Organizer
      Circuit Construction in the Mammalian Brain
    • Place of Presentation
      国立遺伝学研究所(静岡県三島市)
    • Year and Date
      2015-12-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Germline mutation rates and mutation accumulation lines in mice2015

    • Author(s)
      内村 有邦
    • Organizer
      第29回国際哺乳類ゲノム会議
    • Place of Presentation
      横浜市開港記念会館(神奈川県横浜市)
    • Year and Date
      2015-11-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] マウス変異蓄積統計を利用した生殖系列の突然変異率の推定2015

    • Author(s)
      内村 有邦
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学 川内キャンパス内(宮城県仙台市)
    • Year and Date
      2015-09-24
    • Related Report
      2015 Research-status Report
  • [Remarks] 阪大、突然変異マウスを高効率で誕生させることに成功

    • URL

      http://www.zaikei.co.jp/article/20150906/267655.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 蓄積された突然変異が後世代に与える影響の解明に光

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/events/achievement/uchimura-yagi-20150803/

    • Related Report
      2015 Research-status Report
  • [Remarks] 心生物学研究室

    • URL

      http://www.fbs.osaka-u.ac.jp/labs/yagi/index.htm

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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