| Project/Area Number |
15K14369
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MATSUMOTO Takahiro 徳島大学, 大学院医歯薬学研究部(医学系), 准教授 (70447374)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Y染色体 |
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| Outline of Final Research Achievements |
The Y chromosome is male-specific chromosome and considered to be critical for sexual differentiation and male physiology and behavior. However, its physiological impact has been elusive, owing to the lack of a mouse genetic model. The targeted disruption of the Y-linked genes in mouse ES cells has not been successful, because conventional homologous recombination is hampered by the unique structural features of the Y chromosome. We have overcome this problem and generated floxed mouse line, by using a site-directed insertion vector and CRISPR/Cas9 system. Moreover, we developed multi-copy Y gene knockdown transgenic mice with co-cistronic expression of three miRNA hairpins.
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