Analysis of the specific target signaling of glioma stem cells by the integrated phospho-glycomics
| Project/Area Number |
15K14383
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
Araki Norie 熊本大学, 大学院生命科学研究部(医), 准教授 (80253722)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | cancer stem cells / glioma / proteomics |
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| Outline of Final Research Achievements |
Glioma stem cells (GSCs) are considered responsible for the glioma malignancy. To understand the molecular mechanism, we established GSCs having potential to form glioblastoma, and subjected to transcriptome/proteome analyses coupled with phospho-glyco proteomics. The results showed that GSCs had the higher level of O-GlcNAcylation (OG) pathways. Suppressions of OG synthesis resulted in global decreases of OG proteins (OGPs), with the downregulation of GSC proliferations. To identify GSC-associated-OGPs, GSCs were metabolically labeled with GlcNAz and analyzed by LC-MS/MS searching with Ser/Thr phospho-/O-GlcNac-modified peptides. Among 4336 identified-proteins, we focused 23 OGPs possessing the phospho-transition/conversion sites. They involve in DNA-binding, the chromatin remodeling, transcriptions, and neural cell differentiations. The transition/conversion of O-GlcNAcylation to phosphorylation in nuclear factors may be an important mechanism for GSC differentiation to glioma cells.
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Report
(3 results)
Research Products
(51 results)
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[Journal Article] jPOSTrepo: an international standard data repository for proteomes.2017
Author(s)
Okuda S, Watanabe Y, Moriya Y, Kawano S, Yamamoto T, Matsumoto M, Takami T, Kobayashi D, Araki N, Yoshizawa AC, Tabata T, Sugiyama N, Goto S, Ishihama Y.
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Journal Title
Nucleic Acids Res.
Volume: 45
Issue: D1
Pages: D1107-D1111
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin β1 clustering and invasiveness in gastric cancer.2016
Author(s)
Izumi D, Ishimoto T, Miyake K, Sugihara H, Eto K, Sawayama H, Yasuda T, Kiyozumi Y, Kaida T, Kurashige J, Imamura Y, Hiyoshi Y, Iwatsuki M, Iwagami S, Baba Y, Sakamoto Y, Miyamoto Y, Yoshida N, Watanabe M, Takamori H, Araki N, Tan P, Baba H.
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Journal Title
Int J Cancer
Volume: 1;138(5)
Issue: 5
Pages: 1207-19
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] A Novel Data Integration Tool iPEACH Identified the Specific Molecular Networks of Cancer Stem Cells2016
Author(s)
Yamasaki, Y., Nambu,A., Silsirivanit, A., Kobayashi, D., Yoshizawa, A., Kawano, S., Araki, N
Organizer
The 8th Asia Oceania Human Proteome Organization (AOHUPO) Congress
Place of Presentation
Sun moon Lake, Taiwan
Year and Date
2016-09-22
Related Report
Int'l Joint Research
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[Presentation] Lysine propionylation is a novel post-translational modification occurring on many bacterial proteins2016
Author(s)
Okanishi, H., Kobayashi, D., Araki, N., Kim, K., Masui, R., Kuramitsu, S.
Organizer
The 8th Asia Oceania Human Proteome Organization (AOHUPO) Congress
Place of Presentation
Sun moon Lake, Taiwan
Year and Date
2016-09-22
Related Report
Int'l Joint Research
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[Presentation] Analysis of the specific molecular networks of cancer stem cells using a novel data integration tool iPEACH2016
Author(s)
Yamasaki, Y., Nambu,A., Silsirivanit, A., Kobayashi, D., Yoshizawa, A., Kawano, S., Araki, N.
Organizer
15th Human Proteome Organization World Congress (HUPO2016)
Place of Presentation
Taipei, Taiwan
Year and Date
2016-09-18
Related Report
Int'l Joint Research
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[Presentation] A novel post-translational modification: lysine propionylation was identified in bacterial species2016
Author(s)
Okanishi, H., Kobayashi, D., Araki, N., Kim, K., Masui, R., Kuramitsu, S.
Organizer
15th Human Proteome Organization World Congress (HUPO2016)
Place of Presentation
Taipei, Taiwan
Year and Date
2016-09-18
Related Report
Int'l Joint Research
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[Presentation] jPOST: Development of Japan ProteOme STandard Repository/Database2015
Author(s)
Shin Kawano, Yuki Moriya, Shujiro Okuda, Tadashi Yamamoto, Masaki Matsumoto, Daiki Kobayashi, Norie Araki, Akiyasu C. Yoshizawa, Tsuyoshi Tabata, Naoyuki Sugiyama, Susumu Goto, Yasushi Ishihama
Organizer
HUPO2015
Place of Presentation
Vancouver Convention Centre
Year and Date
2015-09-27
Related Report
Int'l Joint Research
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[Presentation] Functional analysis of chloroquine toxicity2015
Author(s)
Diwakar Guragain, Atit Silsirivanit, Wunchana Seubwai, Sopit Wongkham, Ubon Cha’on, Akira Sasao, Ako Sasao, Norie Araki
Organizer
日本プロテオーム学会2015年会
Place of Presentation
熊本森都心プラザ（熊本）
Year and Date
2015-07-22
Related Report
Int'l Joint Research
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