Analysis of the effects of the host environment factors on pancreatic cancer malignancy at whole body levels

• Project/Area Number: 15K14393
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: National Cancer Center Japan
• Principal Investigator: Takahashi Mami 国立研究開発法人国立がん研究センター, 研究所, ユニット長 (90214973)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 膵臓がん / 動物モデル / 宿主環境要因 / 悪性化

Outline of Final Research Achievements

Five culture cell lines which develop well/moderately differentiated carcinoma grafts in C57BL/6J mice were established from pancreatic tumors of Ptfla(Cre/+)/LSL-K-ras(G12D/+) mice. When these cell lines were subcutaneously implanted to obese Ay mice, promotion of tumor growth was observed in 2 cell lines. Orthotopic implantation of the cells develop pancreatic tumor grafts in which marked fatty infiltration was observed, and dissemination to visceral fat. This orthotopic allograft model is useful to study the effects of pancreatic cancer risk factors such as obesity/diabetes on pancreatic tumor growth at whole body levels.

Research Products

• [Journal Article] Activated ductal proliferation induced by N-nitrosobis(2-oxopropyl)amine in fat-induced pancreas of KK-Ay mice. (2018)
  • Author(s): Hori, M., Mutoh, M., Ishigamori, R., Imai, T., Takahashi, M.
  • Journal Title: In Vivo Volume: 32 Issue: 3 Pages: 499-505
  • DOI: 10.21873/invivo.11267
  • Peer Reviewed / Open Access
• [Presentation] Analysis of cancer promotion mechanisms using allograft models of mouse pancreatic adenocarcinoma cell lines (2016)
  • Author(s): 高橋真美、石ヶ守里加子、武藤倫弘、今井俊夫
  • Organizer: 第75回日本癌学会
  • Place of Presentation: パシィフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-10-08
• [Presentation] 肥満モデルAyマウスにおける膵臓の脂肪浸潤のがんの発生・増殖に対する影響 (2016)
  • Author(s): 高橋真美、石ヶ守里加子、武藤倫弘、田中卓二、今井俊夫
  • Organizer: がん予防学術大会2016名古屋
  • Place of Presentation: 名古屋大学基礎医学研究棟（愛知県・名古屋市）
  • Year and Date: 2016-07-01
• [Presentation] Increase of pancreatic cancer development in pancreas-specific K-ras mutant mice by crossing with obesity Ay mice and involvement of the M-CSF (2016)
  • Author(s): 高橋真美、石ヶ守里加子、武藤倫弘、藤井元、宮本真吾、田中卓二、今井俊夫
  • Organizer: 第10回日米癌合同会議
  • Place of Presentation: 米国（ハワイ）
  • Year and Date: 2016-02-19
  • Int'l Joint Research