Role of BRCA2 in the process of estrogen-dependent cell proliferation
Project/Area Number |
15K14394
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Tumor diagnostics
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
MIKI Yoshio 東京医科歯科大学, 難治疾患研究所, 教授 (10281594)
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Co-Investigator(Kenkyū-buntansha) |
中西 啓 東京医科歯科大学, 難治疾患研究所, 准教授 (50321790)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 乳がん / BRCA2 / エストロゲン / エストロゲン受容体 / エストロゲン受容体α |
Outline of Final Research Achievements |
Mutations in the BRCA2 gene are characterized by the predisposition to familial breast and ovarian cancer. BRCA2 cell cycle-dependent expression is associated with binding of the USF, Elf1, and NF-κB transcription factors to the BRCA2 promoter. Recent studies have reported that BRCA2 expression is indirectly elevated in response to estrogen-estrogen receptor (ER) activity, such as binding to transcription factor Sp1 binding sites. In this study, mutations in each of the Sp1, NF-κB, and USF binding sites using site-directed mutagenesis were introduced into the promoter luciferase vector, and estradiol-induced BRCA2 promoter activity and BRCA2 protein level in MCF7 cells was examined. We confirmed two Sp1 sites (-50 to -55 and -69 to -75) responded to estradiol-ER complex activity. These studies aim to elucidate the mechanism underlying breast cancer development by estrogen.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] RAS mutation is a prognostic biomarker in colorectal cancer patients with metastasectomy.2016
Author(s)
Osumi H, Shinozaki E, Suenaga M, Matsusaka S, Konishi T, Akiyoshi T, Fujimoto Y, Nagayama S, Fukunaga Y, Ueno M, Mise Y, Ishizawa T, Inoue Y, Takahashi Y, Saiura A, Uehara H, Mun M, Okumura S, Mizunuma N, Miki Y, Yamaguchi T.
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Journal Title
Int J Cancer
Volume: Epub ahead of print
Issue: 4
Pages: 803-811
DOI
Related Report
Peer Reviewed
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[Journal Article] Prevalence and differentiation of hereditary breast and ovarian cancers in Japan.2015
Author(s)
Nakamura S, Takahashi M, Tozaki M, Nakayama T, Nomizu T, Miki Y, Murakami Y, Aoki D, Iwase T, Nishimura S, Yamauchi H, Ohsumi S, Baba S, Shimizu T.
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Journal Title
Breast Cancer
Volume: 22
Issue: 5
Pages: 462-8
DOI
Related Report
Peer Reviewed
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