| Project/Area Number |
15K14396
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnostics
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| Research Institution | Kochi University |
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Principal Investigator |
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| Research Collaborator |
TSUBOI Makiko
IWASAKI Shinji
OKABAYASHI Takehiro
SHIMA Yasuo
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 腫瘍診断学 / 腫瘍マーカー / 膵癌核酸腫瘍マーカー / 膵臓学 / 膵がん核酸腫瘍マーカー / 膵癌 / エクソソーム / 核酸診断マーカー |
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| Outline of Final Research Achievements |
In vitro experiments suggest that nine mRNAs were concentrated in intracellular exosomes in pancreatic cancer cells, and that these exosome-localized mRNAs were secreted from pancreatic cancer cells. A case-control study that included 20 patients with treatment-naive pancreatic cancer and 30 control individuals without pancreatic diseases was carried out. The nine mRNAs were measured from the purified serum exosomes in this cohort by real-time quantitative RT-PCR. The area under the receiver-operating characteristic curve (AUC) was as same as that of CA19-9 that is the clinical standard tumor biomarker for patients with pancreatic cancer. If some of these mRNAs were combined with CA19-9, the AUC was significantly higher compared with that for CA19-9 alone. Increased expression of serum exosome-localized mRNAs appears highly accurate in diagnosing pancreatic cancer.
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