Functional relationship between mRNA and divergently transcribed lncRNA

• Project/Area Number: 15K14397
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Japanese Foundation for Cancer Research (2016); Sapporo Medical University (2015)
• Principal Investigator: Maruyama Reo 公益財団法人がん研究会, がん研究所, プロジェクトリーダー (60607985)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 長鎖非コードRNA / lncRNA / がん

Outline of Final Research Achievements

Long non-coding RNA (lncRNA) has gained much attention due to its importance in every aspect of cellular and molecular processes. Recently, a lot of studies have reported a list of lncRNA genes whose expressions are dysregulated in cancer, however functions of these lncRNAs are largely unknown. In this study, we focused on pairs of mRNA and divergently transcribed lncRNAs in the same genomic region, and investigated whether there is a functional relationship between mRNA and divergently transcribed lncRNA. We first analyzed public and original expression dataset and finally selected one pair of mRNA and lncRNA for further functional analysis. We found that both the mRNA and the lncRNA have a function related to immune response, however at this point, we haven't have a evidence of functional relationship between them.

Research Products

• [Journal Article] The relationship between EZH2 expression and microRNA-31 in colorectal cancer and the role in evolution of the serrated pathway. (2016)
  • Author(s): Kurihara H, Maruyama R, Ishiguro K, Kanno S, Yamamoto I, Ishigami K, Mitsuhashi K, Igarashi H, Ito M, Tanuma T, Sukawa Y, Okita K, Hasegawa T, Imai K, Yamamoto H, Shinomura Y, Nosho K.
  • Journal Title: Oncotarget Volume: 7 Issue: 11 Pages: 12704-12717
  • DOI: 10.18632/oncotarget.7260
  • Peer Reviewed / Open Access
• [Journal Article] Association of Fusobacterium nucleatum with immunity and molecular alterations in colorectal cancer. (2016)
  • Author(s): Nosho K, Sukawa Y, Adachi Y, Ito M, Mitsuhashi K, Kurihara H, Kanno S, Yamamoto I, Ishigami K, Igarashi H, Maruyama R, Imai K, Yamamoto H, Shinomura Y.
  • Journal Title: World J Gastroenterol Volume: 22 Issue: 2 Pages: 557-566
  • DOI: 10.3748/wjg.v22.i2.557
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of the molecular features of rectal carcinoid tumors to identify new biomarkers that predict biological malignancy. (2015)
  • Author(s): Mitsuhashi K, Yamamoto I, Kurihara H, Kanno S, Ito M, Igarashi H, Ishigami K, Sukawa Y, Tachibana M, Takahashi H, Tokino T, Maruyama R, Suzuki H, Imai K, Shinomura Y, Yamamoto H, Nosho K.
  • Journal Title: Oncotarget Volume: 6 Pages: 22114-22125
  • NAID: 130005161488
  • Peer Reviewed / Open Access
• [Journal Article] Somatic Cell Fusions Reveal Extensive Heterogeneity in Basal-like Breast Cancer. (2015)
  • Author(s): Su Y, Subedee A, Bloushtain-Qimron N, Savova V, Krzystanek M, Li L, Marusyk A, Tabassum DP, Zak A, Flacker MJ, Li M, Lin JJ, Sukumar S, Suzuki H, Long H, Szallasi Z, Gimelbrant A, Maruyama R, Polyak K.
  • Journal Title: Cell Rep Volume: 11 Issue: 10 Pages: 1549-1563
  • DOI: 10.1016/j.celrep.2015.05.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Association of Fusobacterium nucleatum with clinical and molecular features in colorectal serrated pathway. (2015)
  • Author(s): Ito M, Kanno S, Nosho K, Sukawa Y, Mitsuhashi K, Kurihara H, Igarashi H, Takahashi T, Tachibana M, Takahashi H, Yoshii S, Takenouchi T, Hasegawa T, Okita K, Hirata K, Maruyama R, Suzuki H, Imai K, Yamamoto H, Shinomura Y.
  • Journal Title: Int J Cancer Volume: 137 Issue: 6 Pages: 1258-1268
  • DOI: 10.1002/ijc.29488
  • Peer Reviewed
• [Presentation] 胃癌の発生に関与するエピゲノム変化と長鎖非コードRNAの解析 (2015)
  • Author(s): 丸山玲緒、山本英一郎、鈴木拓
  • Organizer: 第74回日本癌学会総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08