Reading cancer specific-metabolism signature from exosome

Research Project

Project/Area Number	15K14400
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Tumor diagnostics
Research Institution	Japanese Foundation for Cancer Research
Principal Investigator	Shiba Kiyotaka 公益財団法人がん研究会, その他部局等, その他 (40196415)
Project Period (FY)	2015-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords	エクソソーム / 細胞外分泌小胞 / P-糖タンパク質
Outline of Final Research Achievements	This research has confirmed that P-glycoprotein, which is known to be involved in multidrug resistance of cancer cell, is expressed on the exosomes that are released from the cells expressing P-glycoprotein. The conditions that allow specific fluorescence labelling of the P-glycoprotein-expressing exosomes using substrates of P-glycoprotein have been sought. However, sticky nature of exosomes under the assay conditions did not allow the establishment of the quantification method.