Development of antigen specific immune cell therapy based on Crispr/Cas9 genome editing system
| Project/Area Number |
15K14404
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor therapeutics
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| Research Institution | Chiba University (2016)
The University of Tokyo (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | CRISPR / Cas9 / ゲノム編集 / がん抗原 / 細胞傷害性T細胞 / Crispr / CRISPR/CAS9 / がん抗原特異的CTL / 抗原特異的な癌免疫細胞療法 |
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| Outline of Final Research Achievements |
We generated T cell receptors-deficient CD8+ T cell delivering modified spCas9 recombinant protein/gRNA complex targeting on T cell receptors by electroporation. We further insert hgp100 specific T cell receptor genes to T cell receptors-deficient CD8+ T cells and generated hgp100-specific CD8+ T cells. These hgp100-specific CD8+ T cells showed good cytotoxic activity against malignant melanoma. We successfully generated the method to delete TCR genes by CRISPR/Cas9 genome editing system and could make antigen-specific cytotoxic T cells by delivering tumor antigen-specific TCR genes to TCR-deficient cells.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] G-CSF-induced sympathetic tone provokes fever and primes antimobilizing functions of neutrophils via PGE2.2017
Author(s)
Kawano Y, Fukui C, Shinohara M, Wakahashi K, Ishii S, Suzuki T, Sato M, Asada N, Kawano H, Minagawa K, Sada A, Furuyashiki T, Uematsu S, Akira S, Uede T, Narumiya S, Matsui T, Katayama Y.
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Journal Title
Blood
Volume: 129
Issue: 5
Pages: 587-597
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Fine Particles Induce Alveolar Macrophage Death and Interleukin-1α Release to Promote Inducible Bronchus-Associated Lymphoid Tissue Formation.2016
Author(s)
Kuroda E, Ozasa K, Temizoz B, Ohata K, Koo CX, Kanuma T, Kusakabe T, Kobari S, Horie M, Morimoto Y, Nakajima S, Kabashima K, Ziegler SF, Iwakura Y, Ise W, Kurosaki T, Nagatake T, Kunisawa J, Takemura N, Uematsu S, Hayashi M, Aoshi T, Kobiyama K, Coban C, Ishii KJ.
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Journal Title
Immunity.
Volume: 45(6)
Issue: 6
Pages: 1299-1310
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Direct Melanoma Cell Contact Induces Stromal Cell Autocrine Prostaglandin E2-EP4 Receptor Signaling that Drives Tumor Growth, Angiogenesis and Metastasis.2015
Author(s)
Inada M, Takita M, Yokoyama S, Watanabe K, Tominari T, Matsumoto C, Hirata M, Maru Y, Maruyama T, Sugimoto Y, Narumiya S, Uematsu S, Akira S, Murphy G, Nagase H, Miyaura C.
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Journal Title
J Biol Chem.
Volume: 290
Issue: 50
Pages: 29781-93
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Roles of mPGES-1, an inducible prostaglandin E synthase, in enhancement of LPS-induced lymphangiogenesis in a mouse peritonitis model.2015
Author(s)
Matsuda H, Hosono K, Tsuru S, Kurashige C, Sekiguchi K, Akira S, Uematsu S, Okamoto H, Majima M.
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Journal Title
Life Sci.
Volume: 142
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Soluble flagellin co-immunization attenuates Th1 priming to Salmonella and clearance by modulating dendritic cell activation and cytokine production.2015
Author(s)
Flores-Langarica A, Bobat S, Marshall JL, Yam-Puc JC, Cook CN, Serre K, Kingsley RA, Flores-Romo L, Uematsu S, Akira S, Henderson IR, Toellner KM, Cunningham AF.
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Journal Title
Eur J Immunol.
Volume: 45
Issue: 8
Pages: 2299-311
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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