Validation of CD271 as a Therapeutic Target of Squamous Cell Carcinoma

• Project/Area Number: 15K14414
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Miyagi Prefectural Hospital Organization Miyagi Cancer Center
• Principal Investigator: TANAKA NOBUYUKI 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 部長 (60280872)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: がん / 免疫抑制 / 癌 / 癌幹細胞

Outline of Final Research Achievements

In order to validate a potential of CD271 as a squamous cell carcinoma target, CD271 was knock-downed by shRNA. CD271 inhibition inhibited both cell proliferation and migration. p42/44Erk inhibition as well as RhoA inhibition inhibited malignant phenotypes of HPCM2 cells. On the other hand, an anti-CD271 Ab did not show significant in vitro effect on the same cells. Together, CD271 is shown to be a potential target for squamous cell carcinoma therapy.

Research Products

• [Journal Article] ESCRT-0 dysfunction compromises autophagic degradation of protein aggregates and facilitates ER stress-mediated neurodegeneration via apoptotic and necroptotic pathways (2016)
  • Author(s): Oshima R, Hasegawa T, Tamai K, Sugeno N, Yoshida S, Kobayashi J, Kikuchi A, Baba T, Futatsugi A, Sato I, Satoh K, Takeda A, Aoki M and Tanaka N
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 24997-24997
  • DOI: 10.1038/srep24997
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method (2016)
  • Author(s): Watanabe K, Fukuhara T, Tsukita Y, Morita M, Suzuki A, Tanaka N, Terasaki H, Nukiwa T, Maemondo M
  • Journal Title: Can Respir J. Volume: 5297329 Pages: 1-6
  • DOI: 10.1155/2016/5297329
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription. (2016)
  • Author(s): Kobayashi EH, Suzuki T, Funayama R, Nagashima T, Hayashi M, Sekine H, Tanaka N, Moriguchi T, Motohashi H, Nakayama K, Yamamoto M.
  • Journal Title: Nat Commun Volume: 7 Issue: 1 Pages: 11624-11624
  • DOI: 10.1038/ncomms11624
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] CD271 regulates the proliferation and motility of hypopharyngeal cancer cells (2016)
  • Author(s): Mochizuki M, Tamai K, Imai T, Sugawara S, Ogama N, Nakamura M, Matsuura K, Yamaguchi K, Satoh K, Sato I, Motohashi H, Sugamura K, Tanaka N
  • Journal Title: Sci Reｐ Volume: 6 Issue: 1 Pages: 30707-30707
  • DOI: 10.1038/srep30707
  • Peer Reviewed / Open Access
• [Journal Article] Enhanced expression of semaphorin 3E is involved in the gastric cancer development. (2016)
  • Author(s): Maejima R, Tamai K, Shiroki T, Yokoyama M, Shibuya R, Nakamura M, Yamaguchi K, Abue M, Oikawa T, Noguchi T, Miura K, Fujiya T, Sato I, Iijima K, Shimosegawa T, Tanaka N, Satoh K.
  • Journal Title: Int J Oncol. Volume: 49(5) Issue: 3 Pages: 619-28
  • DOI: 10.3892/ijo.2016.3593
  • Peer Reviewed / Open Access
• [Journal Article] Xenotransplantation elicits salient tumorigenicity of adult T-cell leukemia-derived cells via aberrant AKT activation. (2016)
  • Author(s): Yamaguchi K, Takanashi T, Nasu K, Tamai K, Mochizuki M, Satoh I, Ine S, Sasaki O, Satoh K, Tanaka N, Harigae H, Sugamura K.
  • Journal Title: Cancer Sci. Volume: ePub Issue: 5 Pages: 638-643
  • DOI: 10.1111/cas.12921
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Induction of Cell Death by Human T-cell Leukemia Virus Type 1 Tax in a Cell Cycle-Dependent Manner. (2016)
  • Author(s): Mizuguchi M, Sasaki Y, Hara T, Higuchi M, Tanaka Y, Funato N, Tanaka N, Fujii M, Nakamura M.
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: 0148217-0148217
  • DOI: 10.1371/journal.pone.0148217
  • Peer Reviewed / Open Access
• [Presentation] Nrf2 plays a pivotal role in Staphylococcus aureus infection control through NLRP3 inflammasome activation and pyroptosis (2016)
  • Author(s): Fukushima M, Nagashima R, Motohash H, Tanaka N
  • Organizer: 日本免疫学会
  • Place of Presentation: 沖縄県
  • Year and Date: 2016-12-05
• [Presentation] 頭頸部癌においてCD271は増殖・浸潤を制御する (2016)
  • Author(s): 望月麻衣 玉井恵一 今井隆之 小鎌直子 中村真央 松浦一登 山口臺範 佐藤賢一 佐藤郁郎 菅村和夫 田中伸幸
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-12-02
• [Presentation] CD271 defines a cancer initiating cell population in hypopharyngeal cancer (2015)
  • Author(s): Nobuyuki Tanaka, Takayuki Imai, Mai Mochizuki, Sayuri Sugawara, Keiichi Tamai, Kazunori Yamaguchi, Kennichi Satoh and Kazuo Sugamura
  • Organizer: AACR2015
  • Place of Presentation: 米国ペンシルバニア州フィラデルフィア
  • Year and Date: 2015-04-18
  • Int'l Joint Research