| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we have attempted to develop a method to detect pathogens in patients having unknown fevers. Even though we were not able to develop the method based on the random-primer amplification due to the high-level noises, we successfully develop the method, by which representative viral, bacterial and parasite pathogens, such as dengue viruses, 16S rRNA sequencing for pan-bacterial detections, and malaria parasites, respectively, could be robustly detected. We actually applied the developed method to diagnose the feverish patients in Manado, Indonesia. We have reported the successful diagnosis from more than 50 cases regarding their infecting pathogens. Moreover, it was possible to identify the SNPs in their genomes. Especially, we found that the SNPs residing in the drug-resistance related genes should have particularly important clinical relevance, when the therapeutic strategy of the patients should be decided.
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