Novel Microbial Response for Critical Metals: Genetic and Computational Analyses on the Metal-Dependent Stimulation using Metatranscriptomics in Deep-Subseafloor Biosphere
| Project/Area Number |
15K14419
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Kochi University |
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Principal Investigator |
Ashiuchi Makoto 高知大学, 教育研究部総合科学系生命環境医学部門, 教授 (20271091)
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| Co-Investigator(Kenkyū-buntansha) |
若松 泰介 高知大学, 教育研究部総合科学系生命環境医学部門, 講師 (60597938)
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| Co-Investigator(Renkei-kenkyūsha) |
INAGAKI Fumio 独立行政法人 海洋研究開発機構, 高知コア研究所, 上席研究員 (50360748)
MORONO Yuki 独立行政法人 海洋研究開発機構, 高知コア研究所, 主任研究員 (30421845)
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| Research Collaborator |
HAKUMAI Yuichi 愛媛大学, 連合農学研究科
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 深海底 / 難培養性微生物 / メタゲノム / レアメタルバイオロジー / 核酸スイッチ / 金属イオン吸着 / 機能性バイオ高分子 |
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| Outline of Final Research Achievements |
Microbes are found throughout nature, the majority of which, however, are unculturable or quite difficult to be multiplied on traditional microbiology. Metagenomics has emerged to access the genetics and physiology of uncultured lives. Here we succeeded in newly identifying critical metals-responsive gene fragments using functional metatranscriptomics in deep-subseafloor biosphere, namely the substrate-induced gene expression screening. The computational analysis revealed riboswitch-like secondary structures in the nucleotide sequences of fragments identified. Such molecular responses for rare earth elements were also found in Bacillus megaterium, a soil bacterium thriving in a vast range of environmental conditions, e.g. in compost to deep-subseafloor. The paper also discussed its survival strategies for cell proliferation and conditional adaptation in the presence of heavy metals including lanthanoids.
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Report
(4 results)
Research Products
(63 results)
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[Presentation] MutS5はHoliday junction DNAなど分岐鎖DNAと強く結合する2017
Author(s)
大下 紘貴, 福井 健二, 佐藤 瑞希, 森澤高至, 溝渕早紀, 美濃部 亜衣, 白米優一, 諸野祐樹, 稲垣史生, 矢野 貴人, 芦内 誠, 若松泰介
Organizer
日本蛋白質科学会 年会
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[Presentation] 南海トラフ深海底コア試料を対象とした基質誘導型遺伝子発現解析法SIGEXによるNi<2+>, Ga<3+>応答遺伝子の同定2016
Author(s)
森澤高至, 諸野祐樹, 寺田武志, 西川聡美, 尾崎和弘, 北村 萌, 大下紘貴, 佐藤瑞希, 稲垣史生, 芦内 誠, 若松泰介
Organizer
日本農芸化学会 2016年度中四国支部大会
Place of Presentation
高知大学朝倉キャンパス（高知県）
Year and Date
2016-09-15
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