| Project/Area Number |
15K14422
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHINO Fumitoshi 東京医科歯科大学, 難治疾患研究所, 教授 (60159754)
RI Jiyon 東京医科歯科大学, 難治疾患研究所, 講師 (20402860)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | レトロウイルス / レトロトランスポゾン / 獲得遺伝子 / GAGタンパク質 / 脳 / Gagタンパク質 / 内在性レトロウィルス / Gag / ヒトゲノム / ORF / 霊長類 |
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| Outline of Final Research Achievements |
Approximately 8% of the human genome consists of LTR retrotransposons and endogenous retroviruses (HERVs). Most are inactivated by heavy mutations and do not function as the retrotransposons nor retoroviruses. However, substantial numbers of short open reading frames (ORFs) from their encoding proteins, such as Gag, Pol and Env, remain and exhibit high conservation among humans, chimpanzees and gorillas. Therefore, we hypothesize that some of such ORFs play some function in these species as Hominidae-specific genes. We screened such putative HERV-derived genes from human genome and made specific antibodies to some candidates. We have been trying to confirm their protein expression in a HEK293 cell line, cerebrum, cerebellum and thalamus.
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