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Development of intracellular pH regulation and profiling of cellular pH response

Research Project

Project/Area Number 15K14436
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field System genome science
Research InstitutionUniversity of Shizuoka (2016)
Kobe University (2015)

Principal Investigator

Hara Kiyotaka  静岡県立大学, 食品栄養科学部, 准教授 (40434378)

Research Collaborator HIRONO Yoko (HARA Yoko)  
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
KeywordsATP / 酵母 / 液胞 / 光 / デルタロドプシン / pH / ミトコンドリア / ロドプシン / バイオテクノロジー
Outline of Final Research Achievements

Here, we described the functional expression of delta rhodopsin which is a light-powered proton pump in intracellular organelle of Saccaromyces cerevisiae to develop the intracellular pH control technology. By fusing delta rhodopsin with a mitochondria-localizing protein, it was redirected to the mitochondria. This mitochondria-localized light-driven pump created a proton motive force and produced more ATP through mitochondrial ATP synthase.
This study showed that we become to be able to control proton transportation activity through mitochondrial inner membrane by using light energy.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2016 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems2016

    • Author(s)
      Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K.Y., Shimatani, Z. and Kondo, A.
    • Journal Title

      Science

      Volume: 353 Issue: 6305 Pages: 6305-6305

    • DOI

      10.1126/science.aaf8729

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Book] 生物の科学 遺伝2016

    • Author(s)
      原清敬
    • Total Pages
      5
    • Publisher
      NTS出版
    • Related Report
      2016 Annual Research Report
  • [Remarks]

    • URL

      http://sweb.u-shizuoka-ken.ac.jp/~env-bioeng/research.html

    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2022-12-28  

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