Development of intracellular pH regulation and profiling of cellular pH response

• Project/Area Number: 15K14436
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: University of Shizuoka (2016); Kobe University (2015)
• Principal Investigator: Hara Kiyotaka 静岡県立大学, 食品栄養科学部, 准教授 (40434378)
• Research Collaborator: HIRONO Yoko (HARA Yoko)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: ATP / 酵母 / 液胞 / 光 / デルタロドプシン / pH / ミトコンドリア / ロドプシン / バイオテクノロジー

Outline of Final Research Achievements

Here, we described the functional expression of delta rhodopsin which is a light-powered proton pump in intracellular organelle of Saccaromyces cerevisiae to develop the intracellular pH control technology. By fusing delta rhodopsin with a mitochondria-localizing protein, it was redirected to the mitochondria. This mitochondria-localized light-driven pump created a proton motive force and produced more ATP through mitochondrial ATP synthase.
This study showed that we become to be able to control proton transportation activity through mitochondrial inner membrane by using light energy.

Research Products

• [Journal Article] Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems (2016)
  • Author(s): Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K.Y., Shimatani, Z. and Kondo, A.
  • Journal Title: Science Volume: 353 Issue: 6305 Pages: 6305-6305
  • DOI: 10.1126/science.aaf8729
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Book] 生物の科学 遺伝 (2016)
  • Author(s): 原清敬
  • Total Pages: 5
  • Publisher: NTS出版
• [Remarks]
  • URL: http://sweb.u-shizuoka-ken.ac.jp/~env-bioeng/research.html