| Project/Area Number |
15K14440
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservation of biological resources
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
檜垣 彰吾 立命館大学, 薬学部, 助教 (70595256)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAI Noriyoshi 国立遺伝学研究所, 系統生物研究センター, 准教授 (50202081)
IMAMURA Masanori 京都大学, 霊長類研究所, 助教 (80567743)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 生殖細胞分化 / 固有魚種 / in vitro 分化 / 卵原細胞 / ホンモロコ / 琵琶湖 / 琵琶湖固有種 / in vitro培養 / 卵子形成 / n vitro培養 / 卵子培養 |
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| Outline of Final Research Achievements |
Honmoroko (Gnathopogon caerulescens) is a small cyprinid endemic to ancient Lake Biwa in Japan and is listed as a critically endangered species. As we have already established cryopreservation of spermatogonia and in vitro differentiation to functional sperm in this fish, we investigated the cryopreservation of oogonia and in vitro differentiation to ova to restore this fish even after its extinction.
We found that oogonia can be efficiently cryopreserved using DMSO, and the culture condition, which enables proliferation of cryopreserved oogonia. In addition, the oocytes isolated from non-spawning female grew in size and released into the medium with prolonged culture. These results suggest that we succeeded to establish cryopreservation and in vitro differentiation of oogonia in G. caerulescens. The diameter of oocytes, however, is smaller than that of the mature ova and no chorion formation was observed, suggesting the culture need to be improved to support oocyte maturation.
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